We narrowed to 73,340 results for: LAS

1. pCMV-SARS-CoV-2 Nsp3TM

   Plasmid

   #204206

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Use

   Tags

   FLAGx1

   Expression

   Mammalian

   Mutation

   none, from obtained clone

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG HDAC5 S259A/S498A

   Plasmid

   #32216

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser259Ala, Ser498Ala

   Promoter

   CMV

   Available since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pLdCH

   Plasmid

   #84291

   Purpose

   Express gRNA and Cas9 in Leishmania with Hygromycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

   Plasmid

   #192273

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MO70-mJP-4

   Plasmid

   #79598

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

   Insert

   Junctophilin-4 (Jph4 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

6. VVT1

   Plasmid

   #65779

   Purpose

   The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SaCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP3-bio

   Plasmid

   #47731

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP3
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2-bio

   Plasmid

   #47710

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP2
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA406

   Plasmid

   #216070

   Purpose

   Fragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistance

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   P2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP790_αGCN4-EZH2-FL-CO

    Plasmid

    #211784

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-KRAB
    

    Use

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE

    Plasmid

    #67295

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Brn2 (Pou3f2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT9751-BEAR-mCherry-preedited

    Plasmid

    #162993

    Purpose

    BEAR control plasmid with split mCherry and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mCherry split with an intron between amino acids 119-120
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAd GFP HDAC5 S259A/S498A

    Plasmid

    #32218

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Adenoviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S259A/S498A

    Promoter

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

14. MSP7-bio

    Plasmid

    #47735

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP7
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE

    Plasmid

    #67292

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Myt1L (Myt1l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-Lmo2

    Plasmid

    #60666

    Purpose

    PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Lmo2 (Lmo2 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. bio-CD4d3+4-ADL1

    Plasmid

    #73090

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ADL1 with bio-rat CD4d3+4

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ADL1 (NCEH1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDECKO-mCherry MALAT1_Enhancer.1

    Plasmid

    #78536

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT-9208

    Plasmid

    #124221

    Purpose

    Cloning plasmid for a self-targeting gRNA library

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKII-nirButterfly

    Plasmid

    #136591

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded voltage indicator nirButterfly

    Depositor

    Vladislav Verkhusha

    Article

    Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

    Insert

    nirButterfly
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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