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pCMV5-Flag-EPLIN beta delta C term

Plasmid

#40931

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

C terminus deleted

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pPN013

Plasmid

#91587

Purpose

Express sgRNA targeting human CSMD1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CSMD1 (CSMD1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

Plasmid

#154770

Purpose

plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in GFP reporter, G1 PylT with A41AA and C5…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM mGata3

Plasmid

#25796

Depositor

Douglas Engel

Article

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

Gata3 (Gata3 Mouse)

Expression

Bacterial

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pMX-Flag-WIP 460 deletion

Plasmid

#11773

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP aa1-460 (WIPF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

aa461-503 deleted

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-TAL-NC2

Plasmid

#43855

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pX61

Plasmid

#46852

Purpose

TOP Hupki-puro2A-Hupki-wt plasmid

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 wild type (TP53 Human, Mouse)

Expression

Mammalian

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pPN177

Plasmid

#91628

Purpose

Express sgRNA targeting human IDO2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

IDO2 (IDO2 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-EGFP-THAP11-rescue-3xFLAG

Plasmid

#37000

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 (THAP11 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Mutations were generated at codons 273-275 conver…

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS

Plasmid

#43969

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Gal4/DBD

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pME-2PH-PLCd

Plasmid

#71283

Purpose

Multisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-delta

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLC delta (Human)

Use

Gateway multisite middle entry clone

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSR04

Plasmid

#69151

Purpose

read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

eef-1A.1 (eft-3) and rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-IA

Plasmid

#61671

Purpose

Non-fluorescent, truncated kinesin-1 fused to IA linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-IA (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBJ1 neo-mo FceRI beta

Plasmid

#8604

Depositor

Jean Kinet

Article

Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

Insert

mouse Fc epsilon RI beta cDNA (Ms4a2 Mouse)

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
mlp-hCHES1sh

Plasmid

#51845

Purpose

shRNA against CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

shRNA against CHES1 (FOXN3 Human)

Use

Retroviral

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pWZL-rCHES1

Plasmid

#51847

Purpose

encodes a CHES1 mRNA that is resistant to shRNA against CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N54

Plasmid

#40934

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-54

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N112

Plasmid

#40935

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-112

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N173

Plasmid

#40936

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-173

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
delta-LIM Isl1

Plasmid

#23261

Depositor

Samuel Pfaff

Article

Thaler et al Cell. 2002 Jul 26. 110(2):237-49.

Insert

Isl1 (Isl1 Rat)

Use

Avian/xenopus/zebrafish

Tags

Lamda-dimer domain

Expression

Mammalian

Mutation

Delta LIM

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only