We narrowed to 14,304 results for: TIM;

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pDONR221-SLC38A1_STOP

Plasmid

#161261

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A1 (SLC38A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiCRISPRv2_mSTING_gRNA_2

Plasmid

#196626

Purpose

Knock-out STING in murine cells, gRNA 2.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING gRNA 2 (Sting1 Mouse)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PINK1 gRNA (BRDN0001147554)

Plasmid

#78035

Purpose

3rd generation lentiviral gRNA plasmid targeting human PINK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PINK1 (PINK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpRY-P2A-EGFP (RTW4830)

Plasmid

#139989

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-UNC93B1_STOP

Plasmid

#161464

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UNC93B1 (UNC93B1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pT3TS-zSpG

Plasmid

#179316

Purpose

In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

Depositor

Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Zebrafish codon-optimized Cas9 variant SpG

Use

CRISPR; In vitro transcription by t3 promoter

Tags

SV40-NLS

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_gRNA_1

Plasmid

#196625

Purpose

Knock-out STING in murine cells, gRNA 1.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING gRNA 1 (Sting1 Mouse)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-zSpRY

Plasmid

#179317

Purpose

In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

Depositor

Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Zebrafish codon-optimized Cas9 variant SpRY

Use

CRISPR; In vitro transcription by t3 promoter

Tags

SV40-NLS

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC20A1_STOP

Plasmid

#161176

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC20A1 (SLC20A1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pClneoMyc-LATS1

Plasmid

#66851

Purpose

Express Myc-tagged LATS1 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

LATS1 (LATS1 Human)

Tags

Myc

Expression

Mammalian

Mutation

P44L mutation in LATS1 compared to GenBank refere…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-XPR1_STOP

Plasmid

#161472

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

XPR1 (XPR1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC34A2_STOP

Plasmid

#161254

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC34A2 (SLC34A2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC34A3_STOP

Plasmid

#161242

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC34A3 (SLC34A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC22A6_STOP

Plasmid

#161131

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A6 (SLC22A6 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MAPK14 gRNA (BRDN0001487162)

Plasmid

#77925

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK14 (MAPK14 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PINK1 gRNA (BRDN0001145357)

Plasmid

#78036

Purpose

3rd generation lentiviral gRNA plasmid targeting human PINK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PINK1 (PINK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SLC7A13_STOP

Plasmid

#161327

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A13 (SLC7A13 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDK1 gRNA (BRDN0001147731)

Plasmid

#77388

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK1 (CDK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-2dV-Camui (wt)

Plasmid

#220366

Purpose

Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

Depositor

Ryohei Yasuda

Article

Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

Insert

mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)

Tags

mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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MASTL gRNA (BRDN0001148591)

Plasmid

#75989

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MASTL (MASTL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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