We narrowed to 27,750 results for: CRISPR-Cas9

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pSpCas9 (BB)-2A-GFP-RB1sg

Plasmid

#229857

Purpose

single guide RNA targeting human RB1 gene

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

RB1sgRNA (RB1 Human)

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP

Plasmid

#192287

Purpose

Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4 (GBX2, CDX2, CDX1, CDX4 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

Plasmid

#202555

Purpose

Synaptotagmin-1 sgRNA2 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

Plasmid

#202556

Purpose

Synaptotagmin-1 sgRNA3 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

Plasmid

#202554

Purpose

Synaptotagmin-1 sgRNA1 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-7SK-Blast

Plasmid

#183620

Purpose

lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

Depositor

Bill Sugden

Article

Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Modified 7SK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-FUSN-mut

Plasmid

#200970

Purpose

induction of phase-separated condensate mediated by FUSN-IDR mutant

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

FUSNmut

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

15 Tyr-to-Ser substitutions

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP

Plasmid

#192287

Purpose

Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4 (GBX2, CDX2, CDX1, CDX4 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

Plasmid

#202555

Purpose

Synaptotagmin-1 sgRNA2 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

Plasmid

#202556

Purpose

Synaptotagmin-1 sgRNA3 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

Plasmid

#202554

Purpose

Synaptotagmin-1 sgRNA1 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

Plasmid

#188705

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

ST sgRNAs

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT_hifiDn29-dCas9-P2A-GFP

Plasmid

#247168

Purpose

In vitro transcription of human codon optimized fusion of hifiDn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT_superDn29-dCas9-P2A-GFP

Plasmid

#247166

Purpose

In vitro transcription of human codon optimized fusion of superDn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT_goldDn29-dCas9-P2A-GFP

Plasmid

#247167

Purpose

In vitro transcription of human codon optimized fusion of goldDn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-FUSN-mut

Plasmid

#200970

Purpose

induction of phase-separated condensate mediated by FUSN-IDR mutant

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

FUSNmut

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

15 Tyr-to-Ser substitutions

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

Plasmid

#167295

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAK (BAK1 Human)

Expression

Mammalian

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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