We narrowed to 10,212 results for: FIE
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Plasmid#172657PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD10kbA/pegRNA-HD10kbB
UseCRISPRAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-FE-PuroR
Plasmid#186746PurposeModified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpinDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-10-SV40 polyA
Plasmid#190874PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode10
UseAAVMutationWTAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-Sin3a-115-212(pL01)
Plasmid#124386PurposeE.Coli produced SIN3A PAH1 purified by FPLC nickel columnDepositorAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_GFP_sgEPAS1#1
Plasmid#183119PurposepLentiGuide modified to express GFP and an sgRNa targeting EPAS1DepositorInsertsgEPAS1-1
UseLentiviralPromoterU6Available SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_GFP_sgEPAS1#2
Plasmid#183120PurposepLentiGuide modified to express GFP and an sgRNA targeting EPAS1DepositorInsertEPAS1-2
UseLentiviralPromoterU6Available SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-THT III
Plasmid#55791PurposeGATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistanceDepositorTypeEmpty backboneUseRNAi; GatewayExpressionMammalianPromotermodified H1 RNA geneAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3013
Plasmid#91100PurposeModule C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoterPvUbi2Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(0)
Plasmid#164269PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
TagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.1
Plasmid#32467DepositorInsertG-GECO1.1
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAT.011
Plasmid#119555PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmGFP
Plasmid#188451PurposeExpression of a modified GFP (mGFP) with short half-lifeDepositorInsertCyclin destruction box
ExpressionMammalianPromoterCMVAvailable SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST(frt)(ATG_V5-APEX2_noSTOP)
Plasmid#168207PurposeDestination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selectionDepositorTypeEmpty backboneUseUnspecified; Pdest gatewayTagsV5 APEX2ExpressionMammalianPromoterCMVAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVL23 - pVND7
Plasmid#116019Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpVND7
UseUnspecifiedAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW177 - pCASP1
Plasmid#115986Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpCASP1
UseUnspecifiedAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-TGF beta type I receptor (mL45)
Plasmid#34701DepositorInsertTGF beta type I receptor (Tgfbr1 Rat)
TagsFlagExpressionMammalianMutationN265A, D267A, N268APromoterCMVAvailable SinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-SapI
Plasmid#128599PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsmBI
Plasmid#128648PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET:GACG:GFP
Plasmid#60735PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET:GCAT:GFP
Plasmid#60736PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only