We narrowed to 29,721 results for: REP

1. pET-IG-mSA

   Plasmid

   #80706

   Purpose

   Expresses monomeric streptavidin (mSA) in bacterial cells

   Depositor

   Matthieu Sainlos

   Article

   Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

   Insert

   Monomeric streptavidin
   

   Use

   Tags

   10xHis tag and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. double cut NH-donor

   Plasmid

   #83576

   Purpose

   eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   eGFP
   

   Use

   Nhej donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRT802

   Plasmid

   #68113

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   aph11
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BFP Homology Donor

   Plasmid

   #149372

   Purpose

   Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Insert

   CDDR BFP Homology Donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330-Flag-wtSpCas9-H840A

   Plasmid

   #80453

   Purpose

   SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   SpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXGFPC-5 Pxyl-dcas9 (Sth3)

   Plasmid

   #133317

   Purpose

   expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus thermophilus #3)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   xylose

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS-PtH2B

   Plasmid

   #133390

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   No promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GG-EBNA-OCT4

   Plasmid

   #69537

   Purpose

   Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   5 concatenated gRNA transcriptional cassettes targeting OCT4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 2a-copGFP(+HA)

   Plasmid

   #83806

   Purpose

   Express copGFP reporter for HDR-induced knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   copGFP
   

   Use

   Homologous recombination for gapdh loci

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRT801

    Plasmid

    #68112

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid

    #133318

    Purpose

    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus pasteurianus)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    xylose

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV 4xSTAR-mTFP-NLS-blast

    Plasmid

    #136257

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTFP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-PtH

    Plasmid

    #133391

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Tol2 8xSTAR-mScarletI. PGK-puro

    Plasmid

    #136266

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS-BtH

    Plasmid

    #133392

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRPB1-CTD8

    Plasmid

    #112820

    Purpose

    pSMF2 with 8 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRPB1-CTD10

    Plasmid

    #112821

    Purpose

    pSMF2 with 10 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET16b-RPA4/His14 (MSW#949)

    Plasmid

    #208075

    Purpose

    Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA4, RPA14(His-tagged)
    

    Use

    Tags

    10xHis tag on RPA14

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p11d-RPA70/14 (MSW#134)

    Plasmid

    #208076

    Purpose

    Expression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tag

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Insert

    RPA1, RPA3 (RPA1, RPA3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge