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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,602 results for: REP

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Showing: 7101 - 7120 of 31602 results

  3. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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  5. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101 and VanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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  14. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.
    Depositor
    Daniel Voytas
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  19. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available Since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65214-plasmid-map-sequence-id-116341

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  20. pSR646

    Plasmid
    #65215
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-6 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available Since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65215-plasmid-map-sequence-id-112598

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Showing: 7101 - 7120 of 31602 results