We narrowed to 19,029 results for: Tec

1. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

   Plasmid

   #160611

   Purpose

   Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:LbCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. [pSK467] pLJC5 HA-Depdc5

   Plasmid

   #109343

   Purpose

   Lenti-viral expression of HA-tagged wildtype Depdc5

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GRAB_OTmut

   Plasmid

   #185388

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Has Service

   AAV1 and AAV9

   Insert

   GPCR activation based oxytocin control sensor GRAB_OTmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CaTCH BC1

   Plasmid

   #154195

   Purpose

   Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   CaTCH BC1
   

   Use

   Lentiviral; Catch barcode labeling

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaTCH_empty-backbone

   Plasmid

   #157746

   Purpose

   Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral; Catch barcode cloning

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA031

   Plasmid

   #216011

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-SpRY (D10A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA030

   Plasmid

   #216010

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-SpG (D10A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-ms-Sox17

   Plasmid

   #50781

   Purpose

   retroviral expression of mouse Sox17

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6GRNA

   Plasmid

   #68370

   Purpose

   gRNA scaffold with hU6 promoter

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. NO- DSD Sensor 2

    Plasmid

    #199786

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    NO- DSD Sensor
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-OsTIR1(F74A)

    Plasmid

    #140731

    Purpose

    OsTIR1(F74A)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74A)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid

    #191164

    Purpose

    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (additional cutsites)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9 TadCBEa

    Plasmid

    #193782

    Purpose

    Express TadCBEa (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa-SpCas9D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-Cluc-RMA

    Plasmid

    #189624

    Purpose

    Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    Cypridina luciferase fused to Fc
    

    Use

    AAV and Luciferase

    Tags

    Cluc and IgG1 Fc

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-ADARcd-YTHD422N

    Plasmid

    #194402

    Purpose

    ADAR1 catalytic domain E488Q fused to YTHD422N

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    ADARcd fused to YTHD422N (ADAR Human)
    

    Tags

    ADARcd-YTHD422N-HA

    Expression

    Mammalian

    Mutation

    D422N mutation in the YTH domain to enhance m6A b…

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)

    Plasmid

    #140658

    Purpose

    OsTIR1(WT)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT) mAID-EGFP-Nluc TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AXBE

    Plasmid

    #204605

    Purpose

    Mammalian expression of adenine transversion base editor AXBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    S. pyogenes Cas9 (D10A)

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP gRNA (BRDN0000562805)

    Plasmid

    #80035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRT498-OtUBD(V203D)

    Plasmid

    #190090

    Purpose

    Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD(V203D)
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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