We narrowed to 36,940 results for: kan

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pCyterm-mScarlet3_N1

Plasmid

#189778

Purpose

Construct for testing oligimerization of mScarlet3 in mammalian cells by OSER assay

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

Cyterm-mScarlet3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

Plasmid

#196192

Purpose

human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

Depositor

Jürgen Knoblich

Publication

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4

Use

Znf donor

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAF020 p3E-2A-mCerulean

Plasmid

#195974

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCerulean

Use

Other

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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460 p3E-2A-mCherrypA

Plasmid

#195970

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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763 pME mApple

Plasmid

#195960

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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612 p3E-2A-mCherryCAAXpA

Plasmid

#195971

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry-CAAX (membrane)

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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plk1 T210D

Plasmid

#132964

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Publication

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Mutation

T210D

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9a

Plasmid

#169071

Purpose

Express human Rab9a in mammalian cells

Depositor

Gia Voeltz

Publication

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)

Plasmid

#191451

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Publication

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

N-WASP

Expression

Mammalian

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRT498-3xOtUBD

Plasmid

#190088

Purpose

Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Publication

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

3xOtUBD

Tags

6xHis and MBP

Expression

Bacterial

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD

Plasmid

#190089

Purpose

Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Publication

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Tags

6xHis and MBP

Expression

Bacterial

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR-221-P3-P4

Plasmid

#186351

Purpose

Gateway donor vector with AttP3 AttP4 recombination sites

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SUMO-MjDim1

Plasmid

#189936

Purpose

Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

Depositor

Chuan He

Publication

Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

Insert

rsmA (MJ_RS00455 Methanocaldococcus jannaschii)

Tags

6x His tag, FLAG tag, SUMO tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET30a(+)-PE2

Plasmid

#180015

Purpose

Expressing PE2 protein in E.coli for protein purification

Depositor

Dirk Hockemeyer

Publication

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2

Tags

6xHis

Expression

Bacterial

Promoter

Lac operator-controlled T7 promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Integrin Beta1-mPA-GFP

Plasmid

#186302

Purpose

Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

Depositor

Pakorn Kanchanawong

Publication

Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

Insert

Integrin Beta 1 (ITGB1 Human)

Tags

mPA-GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-monSTIM1

Plasmid

#161795

Purpose

Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Publication

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

MonSTIM1 (STIM1 Mustard Weed, Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pKL2315

Plasmid

#187876

Purpose

Expresses Cre recombinase.

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

J23107

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lamp1-mCherry-FRB

Plasmid

#186576

Purpose

Expression of mCherry-FRB at the outer surface of the lysosome using LAMP1

Depositor

Stephen Royle

Publication

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

LAMP1 (LAMP1 Human)

Tags

mCherry-FRB

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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σ3-mCherry

Plasmid

#186580

Purpose

Expression of adaptor protein 3 (AP3) sigma subunit AP3S1 tagged with mCherry

Depositor

Stephen Royle

Publication

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP3S1 (AP3S1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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6xAP-1_RE::Renilla

Plasmid

#178317

Purpose

AP-1 Renilla luciferase reporter

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

AP-1 Renilla Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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