We narrowed to 14,727 results for: EGF

1. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

   Plasmid

   #140005

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

   Plasmid

   #197502

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

   Plasmid

   #176280

   Purpose

   Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mNaChBac-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   human Synapsin I

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

   Plasmid

   #176278

   Purpose

   Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   Kir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   human Synapsin I

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

   Plasmid

   #51509

   Purpose

   Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV1

   Insert

   tdTomato-T2A-SypEGFP
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

   Plasmid

   #197501

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

   Plasmid

   #223113

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-EGFP-8X2C (AAV GABA mAGNET)

   Plasmid

   #114214

   Purpose

   AAV vector that targets expression to GABAergic inhibitory neurons in rodent cortex with 98% selectivity

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   AAV and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   human synapsin (hSyn)

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

   Plasmid

   #176279

   Purpose

   Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   GYG to AAA (aa144-146)

   Promoter

   human Synapsin I

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid

    #197507

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

    Plasmid

    #63729

    Purpose

    To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

    Depositor

    Mark Kay, Joseph Wu

    Article

    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

    Inserts

    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    SFFV

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid

    #197500

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid

    #185918

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-Blast

    Plasmid

    #205557

    Purpose

    Express EPN24-PCP-IRES-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-PCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid

    #223114

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

    Plasmid

    #199781

    Purpose

    Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

    Depositor

    Sascha Martens

    Insert

    NEMO (IKBKG Human)
    

    Tags

    GST-Thrombin-TEV-EGFP

    Expression

    Bacterial

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid

    #197504

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid

    #197508

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

    Promoter

    CMV and T7

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRIP-SFFV-EGFP-FLAG-cGAS E225A/D227A

    Plasmid

    #127662

    Purpose

    Over-express catalytically inactive EGFP-FLAG-cGAS E225A/D227A

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Mutation

    E225A/D227A

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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