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I-AAAC

Plasmid

#42964

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-AAAC

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
I-AAAG

Plasmid

#42965

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-AAAG

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
I-AAAT

Plasmid

#42966

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-AAAT

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
I-AACC

Plasmid

#42968

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-AACC

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
I-AACG

Plasmid

#42969

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-AACG

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
I-AACT

Plasmid

#42970

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-AACT

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
I-CAAG

Plasmid

#43029

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-CAAG

Use

Bacterial cloning

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
III-GCCA

Plasmid

#43623

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-GCCA

Use

Bacterial cloning

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a I18A

Plasmid

#40043

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a I18A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Isoleucine 18 to Alanine

Promoter

CMV promoter

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a linker

Plasmid

#40040

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a linker (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

linker domain (144-353)

Promoter

cmv

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pCE-mp53DD

Plasmid

#41856

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp53 (Trp53 Mouse)

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pMIR-FLAG-MLL-AF9

Plasmid

#71444

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-AF9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMIG-FLAG-MLL-AF9

Plasmid

#71443

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-Af9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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loxP-G418-LoxP-TurboID-Rab11 HR

Plasmid

#230026

Purpose

Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Article

Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

Insert

genomic sequence Rab11 locus (RAB11A Human)

Use

CRISPR

Tags

Turbo-ID, V5

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pIG-601_NY-ESO-1_TCR_GFP

Plasmid

#207482

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

GFP, NY-ESO-1_TCR

Use

CRISPR

Mutation

Potential silent point mutation in GFP sequence

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2_SEPT6

Plasmid

#174497

Purpose

bacterial co-expression of human SEPT2 and of human SEPT6

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN6 (SEPTIN6 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10

Plasmid

#130883

Purpose

Expresses human RAB10 with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTagBFP2-blast

Plasmid

#125239

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTagBFP2 (codon optimized)

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

Plasmid

#197044

Purpose

In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

Depositor

Liqun Luo

Article

Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

Inserts

pCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA

Tags

HA, myc

Expression

Mammalian

Promoter

pCAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-tet-iCas9-BFP2

Plasmid

#125519

Purpose

all-in-one-inducible Cas9

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Insert

AAVS1-TET-ON 3G-SpCas9-P2A-BFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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