We narrowed to 24,851 results for: ina
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gTBEv3
Plasmid#220619Purposevector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInserthUNG2-nSpCas9
ExpressionMammalianMutationhUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…PromoterhU6, EF1aAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)dTomato
Plasmid#200113PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
ExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence, no other pr…Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ADARcd-YTHD422N
Plasmid#194402PurposeADAR1 catalytic domain E488Q fused to YTHD422NDepositorInsertADARcd fused to YTHD422N (ADAR Human)
TagsADARcd-YTHD422N-HAExpressionMammalianMutationD422N mutation in the YTH domain to enhance m6A b…Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor
Plasmid#193469PurposeCp36 Donor with promoter for mcherry and puro expressionDepositorInsertCp36_attP
ExpressionMammalianAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -2
Plasmid#180009PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo WT nsp1
Plasmid#175412PurposeExpressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA
Plasmid#113686PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.DepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Cre/LoxTagsKRABAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Epac1 SAAX
Plasmid#118313PurposeExpression of human Epac1DepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3A
Plasmid#66819PurposedCas9 fused to BFP and the human DNMT3A catalytic domainDepositorInsertdSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
TagsdSpCas9-BFP-DNMT3AExpressionMammalianAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
CDK4-luciferase full length (2135)
Plasmid#86656PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation2120 base pairs including up to coding nucleotide…PromoterCDK4Available SinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(1-555)
Plasmid#90105PurposeMammalian expression of Flag-MCPIP1(1-555)DepositorAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAPK3
Plasmid#73235PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK3 (MAPK3 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00069:Pkinase 42-330PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-Farnesyl-5
Plasmid#57717PurposeLocalization: Membrane, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertFarnesyl (HRAS Human)
TagsDendra2ExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable SinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTurquoise-Farnesyl-5
Plasmid#55551PurposeLocalization: CAAX - Membrane, Excitation: 434, Emission: 474DepositorInsertFarnesyl (HRAS Human)
TagsmTurquoiseExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTFP1-TOMM20-N-10
Plasmid#55511PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 462, Emission: 492DepositorAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
myc Rif-TN
Plasmid#38769DepositorInsertRif (RHOF Human)
TagsmycExpressionMammalianMutationT33N dominant-negative mutationPromoterCMVAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-p110γ
Plasmid#34892DepositorInsertp110γ (PIK3CG Human)
UseAdaptor plasmidAvailable SinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mRor2deltaKringle
Plasmid#22630DepositorInsertRor2 delta Kringle (Ror2 Mouse)
TagsFlagExpressionMammalianMutationDeletion of Kringle domainAvailable SinceNov. 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
PKC epsilon dNPS
Plasmid#21241DepositorAvailable SinceNov. 10, 2009AvailabilityAcademic Institutions and Nonprofits only
