We narrowed to 4,407 results for: BRE

1. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

   Plasmid

   #157701

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBT-RP8.1

   Plasmid

   #189846

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   mRFP and tagBFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

   Plasmid

   #157699

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

   Plasmid

   #157693

   Purpose

   mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. EGFP-Mm_MyoGEF(WT)

   Plasmid

   #136325

   Purpose

   Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   PLEKHG6 (Plekhg6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCV-BRAF-WT-IRES-mCherry

   Plasmid

   #221027

   Purpose

   Fluorescent reporter for expressing a segment of wild type BRAF

   Depositor

   Mohamed Fareh

   Article

   Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

   Insert

   BRAF wildtype (BRAF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(+)GαsS-246-Nluc

   Plasmid

   #207157

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(246)N

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)GαsL-260-NLuc

   Plasmid

   #207158

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(260)N

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pb-miRE-tre-3xFLAG-hnRNPU

   Plasmid

   #196087

   Purpose

   Expresses mouse hnRNPU with a 3xFLAG tag in mammalian cells

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAF-A (Hnrnpu Mouse)
   

   Use

   Tags

   N-terminal 3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CAGGS-safb∆sap-NLS-3XFLAG-V5

    Plasmid

    #196091

    Purpose

    Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Removed amino acids 31-65 (SAP domain) of mouse S…

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAGGS-safb∆rrm-NLS-3XFLAG-V5

    Plasmid

    #196093

    Purpose

    Expresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Removed amino acids 428-506 (RRM) of mouse SAFB p…

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMeCP2G pc1121

    Plasmid

    #167566

    Purpose

    The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

    Depositor

    Cristina Cardoso

    Article

    Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

    Inserts

    Methyl-CpG binding protein 2 (Mecp2 Rat)
    GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGBT-RP2.2

    Plasmid

    #189842

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBT-RP2.3

    Plasmid

    #189843

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBT-RP8.3

    Plasmid

    #189848

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    mRFP and tagBFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. A1-LCD-10R

    Plasmid

    #178664

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues removed (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-10R (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    ten Arg residues removed

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. A1-LCD+8D

    Plasmid

    #178674

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with eight Asp residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+8D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    eight Asp residues added

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. A1-LCD+7R

    Plasmid

    #178667

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+7R (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    seven Arg residues added

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. A1-LCD-2K

    Plasmid

    #178668

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with two Lys residues removed (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-2K (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    two Lys residues removed

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. A1-LCD+4D

    Plasmid

    #178673

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues added (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+4D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    four Asp residues added

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge