We narrowed to 6,495 results for: Gca

1. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104493

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV Retrograde and AAV1

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104497

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

   Plasmid

   #118273

   Purpose

   pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV Cag Flex H2B Gcamp6f reverse

   Plasmid

   #74155

   Purpose

   Calcium Sensor

   Depositor

   Loren Looger

   Insert

   Gcamp6f
   

   Use

   AAV

   Tags

   H2B

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck

   Plasmid

   #58524

   Purpose

   Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   sdChR(C138S, E154A)-TS-GCaMP6f-ER
   

   Use

   Lentiviral

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   changed Cysteine 138 to Serine; changed Glutamate…

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV Cag Flex H2B Gcamp6s reverse

   Plasmid

   #74157

   Purpose

   Calcium Sensor

   Depositor

   Loren Looger

   Insert

   Gcamp6s
   

   Use

   AAV

   Tags

   H2B

   Expression

   Mutation

   Promoter

   SpeI

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

   Plasmid

   #63886

   Purpose

   An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized GCaMP3(wt)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human SYN1

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

   Plasmid

   #63884

   Purpose

   An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized GCaMP3(wt)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE

   Plasmid

   #105322

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV1

   Insert

   jGCaMP7c variant 1513
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

    Plasmid

    #105323

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7c variant 1513
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218863

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218867

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

    Plasmid

    #68719

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #68717

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cre-dependent expression from inverted open readi…

    Promoter

    CAG-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #68720

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cre-dependent expression from inverted open readi…

    Promoter

    hSyn1-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid

    #218865

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8s
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

    Plasmid

    #159636

    Purpose

    Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE

    Plasmid

    #87912

    Purpose

    Kamicas9 lentiviral vector

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    shHTT1, sgCas9, cherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1, 7SK, PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222858

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CAG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCAG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222860

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(GCA)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgGCA
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge