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[pSK475] pLJC5 HA-Depdc5(A)

Plasmid

#109344

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant A

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

DIYGD(185-189)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK476] pLJC5 HA-Depdc5(B)

Plasmid

#109345

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant B

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

EQPLH(371-375)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK478] pLJC5 HA-Depdc5(P)

Plasmid

#109347

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant P

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

YDLLP(775-779)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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XE157 GFP-XDpr-delta PDZB-CS2+

Plasmid

#16765

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-delta PDZB (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Mutation

XDpr is truncated to delete the last four amino a…

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pK RagA(Q66L)-RagC(Q120L)

Plasmid

#99702

Purpose

Bacterial expression of RagA(Q66L)-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

Q120L and Q66L

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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1287 p3E-mut let-7 etv2 3’ UTR

Plasmid

#49011

Purpose

Zebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

Mutated the Let7 miRNA binding sites

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only
pLX301-KANK1

Plasmid

#121985

Purpose

overexpress of KANK1

Depositor

GuangJun Zhang

Article

Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

Insert

KANK1 (KANK1 Human)

Use

Lentiviral

Promoter

CMV promoter

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

Plasmid

#35500

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Plasmid

#35501

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD2

Plasmid

#170847

Purpose

AAV backbone for Cre-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD1

Plasmid

#170846

Purpose

AAV backbone for transgene expression in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS3

Plasmid

#170854

Purpose

AAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6

Plasmid

#170857

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2

Plasmid

#170853

Purpose

AAV backbone for Cre-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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374 pME-ets1a

Plasmid

#49000

Purpose

full length coding sequence for zebrafish ets1a gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

v-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD3

Plasmid

#170848

Purpose

AAV backbone for Flp-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4

Plasmid

#170849

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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[pSK483] pLJC5 Flag-Depdc5(P)

Plasmid

#109342

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant P

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

YDLLP(775-779)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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375 pME-fli1a

Plasmid

#49001

Purpose

full length coding sequence for zebrafish fli1a gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1a (fli1 Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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