Addgene

We narrowed to 1,127 results for: cas12

Showing: 701 - 720 of 1127 results

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EC70194

Plasmid

#209455

Purpose

Cloning protospacers for Cas9 editing

Depositor

Cristobal Uauy, Wendy Harwood

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

HvU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70190

Plasmid

#209451

Purpose

Cloning protospacers for Cas9 editing

Depositor

Cristobal Uauy, Wendy Harwood

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

HvU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUCsRNAP

Plasmid

#182746

Purpose

Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

Depositor

James Collins

Article

Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

Insert

small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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EC67907

Plasmid

#211794

Purpose

Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

Depositor

Cristobal Uauy, Wendy Harwood

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas9, GR-GRF-GIF

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ11353

Plasmid

#183098

Purpose

pHR-CAG-sfGFP* ABE_3-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

GFP*

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pKEW270-pTet-empty

Plasmid

#115669

Purpose

Vector for anti-CRISPR expression with pTet

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pFH47

Plasmid

#125812

Purpose

Level0 LbCas12a, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKEW302-AcrVA4

Plasmid

#115656

Purpose

Expresses AcrVA4 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pKEW303-AcrVA5

Plasmid

#115657

Purpose

Expresses AcrVA5 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFH17

Plasmid

#125803

Purpose

Level0 LbCas12a, rice codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, rice codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Aa-artsg13 (BsaI)

Plasmid

#188279

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Aa-artsg13

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJK504

Plasmid

#155381

Purpose

dCas12a crRNAs

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

dCas12a oscillator crRNAs

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFH16

Plasmid

#125801

Purpose

Level0 FnCas12a, rice codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

FnCas12a, rice codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMB28

Plasmid

#223011

Purpose

Cas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (I)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFH46

Plasmid

#125811

Purpose

Level0 FnCas12a, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

FnCas12a, human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBE45

Plasmid

#174655

Purpose

CAPTURE method receiver plasmid for Streptomyces heterologous expression

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBE44

Plasmid

#174654

Purpose

CAPTURE method 15A origin of replication receiver plasmid

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBE48

Plasmid

#174656

Purpose

CAPTURE method BAC origin of replication receiver plasmid

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141B-ge4.1

Plasmid

#205144

Purpose

Guide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification

Depositor

Yiping Qi

Article

Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU3

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBE50

Plasmid

#174657

Purpose

CAPTURE method receiver plasmid for Bacillus cloning and heterologous expression

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge