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We narrowed to 1,199 results for: nanoLuc

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Showing: 701 - 720 of 1199 results

  1. pTS2317

    Plasmid
    #169624
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169624-plasmid-map-sequence-id-365608

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  6. G13-CASE

    Plasmid
    #168127
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F125/D126 within GNA13
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168127-plasmid-map-sequence-id-332872

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  8. Gi2-CASE

    Plasmid
    #168121
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at C112/E115 within GNAI2
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168121-plasmid-map-sequence-id-332861

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  9. pcDNA3.1+-Tornado-nLuc

    Plasmid
    #212611
    Purpose
    For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.
    Depositor
    Samie Jaffrey
    Publication
    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tornado-CVB3-nLuc
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212611-plasmid-map-sequence-id-420917

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  10. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168123-plasmid-map-sequence-id-332865

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  11. pcDNA3.1-Gβγ-BERKY3

    Plasmid
    #158219
    Purpose
    BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)
    Depositor
    Mikel Garcia-Marcos
    Publication
    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    158219-plasmid-map-sequence-id-311194

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  12. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168122-plasmid-map-sequence-id-332862

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  16. AIMTOR A757

    Plasmid
    #140829
    Purpose
    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757
    Depositor
    Anne Bonnieu
    Publication
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AIMTOR A757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140829-plasmid-map-sequence-id-305143

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  19. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

    Plasmid
    #113862
    Purpose
    Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Publication
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-NLuc1.1
    12GLI-Fluc
    CBF-GLuc
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113862-plasmid-map-sequence-id-328796

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  20. G15-CASE

    Plasmid
    #168126
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at E244/N245 within GNA15
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168126-plasmid-map-sequence-id-332871

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Showing: 701 - 720 of 1199 results