We narrowed to 10,582 results for: plasmids 101

1. T4 Lysozyme I3S

   Plasmid

   #18212

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I3S

   Promoter

   Available Since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

2. T4 Lysozyme I3T

   Plasmid

   #18213

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I3T

   Promoter

   Available Since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

3. T4 Lysozyme I3V

   Plasmid

   #18214

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I3V

   Promoter

   Available Since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

   Plasmid

   #74101

   Purpose

   vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NanoBRET BiBRET Vector, NanoLuc-HRAS WT-CRAF RBD-HaloTag

   Plasmid

   #236861

   Purpose

   Express NanoLuc(R)-HRAS WT-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   HRAS (HRAS Human)
   cRAF(RBD) (RAF1 Human)
   

   Use

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. NanoBRET BiBRET Vector, NanoLuc-NRAS (Q61K)-CRAF RBD-HaloTag

   Plasmid

   #236860

   Purpose

   Express NanoLuc(R)-NRAS (Q61K)-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   NRAS (NRAS Human)
   cRAF(RBD) (RAF1 Human)
   

   Use

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDest17_EWSR1/ZNF384 fusion protein LCD (1-149)

   Plasmid

   #235000

   Purpose

   Bacterial expression of N-terminally 6His tagged EWSR1/ZNF384

   Depositor

   Tanja Mittag

   Insert

   EWSR1/ZNF384 (Ewing sarcoma RNA binding protein 1/ Zinc finger protein 384) fusion protein (ZNF384, EWSR1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p667-UBC-TLK2-V5_IDG-K

   Plasmid

   #135251

   Purpose

   Gateway destination clone of TLK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TLK2-V5 (TLK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. COL25A1_53-654_AviN_HisC

   Plasmid

   #195880

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   COL25A1 (COL25A1 Human)
   

   Use

   Pfhmsp-avin-lic-c, baculovirus expression

   Tags

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-CMV-Flag-TLK2_IDG-K

    Plasmid

    #135278

    Purpose

    Gateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-TLK2 (TLK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p667-UBC-TLK2-V5-TurboID_IDG-K

    Plasmid

    #135250

    Purpose

    Gateway destination clone of TLK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TLK2-V5-TurboID (TLK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-7aa-cb5-pEGFP-C1

    Plasmid

    #177407

    Purpose

    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p667-UBC-TLK2-V5-miniTurbo_IDG-K

    Plasmid

    #135249

    Purpose

    Gateway destination clone of TLK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TLK2-V5-miniTurbo (TLK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIRESN2_GFP_YTA_Champ1_328-354

    Plasmid

    #105639

    Purpose

    Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP

    Depositor

    Thanos Halazonetis

    Insert

    Champ1 (CHAMP1 Human)
    

    Use

    Tags

    GFP YTA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid

    #107107

    Purpose

    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

    Depositor

    Michael E. Wright

    Insert

    human ubiquitin UBB (UBB Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Tet

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. cacna2d1 R241A D491A HA pcDNA3

    Plasmid

    #206109

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding

    Depositor

    Annette Dolphin

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R241A D491A

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AASS (AASSA-c013)

    Plasmid

    #98227

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AASSA (AASS Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Expression

    Mutation

    pfam03435:Saccharop_dh 483-916

    Promoter

    polyhedrin

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mouse KANK4 Ankyrin Repeats pET151

    Plasmid

    #164057

    Purpose

    Expresses mouse KANK4 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDZ2FE_Champ1_1-812_2PA

    Plasmid

    #105676

    Purpose

    Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding

    Depositor

    Thanos Halazonetis

    Insert

    Champ1 (CHAMP1 Human)
    

    Use

    Tags

    2X Flag tag

    Expression

    Mammalian

    Mutation

    Mutations: P336A, P341A

    Promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His6-Halo-TEV-Akt2

    Plasmid

    #87606

    Purpose

    expresses His-halo tagged at the N-terminus of human Akt2 protein

    Depositor

    Yimon Aye

    Insert

    AKT 2 (AKT2 Human)
    

    Use

    Zebrafish (danio rerio) expression

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge