-
-
pLenti CMV rtTA3 Hygro (w785-1)
Plasmid#26730Purpose3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, HygroDepositorInsertTetracycline repressor A3 mutant
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSW003-PpsbA-mNeonGreen
Plasmid#205019PurposeBroad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)DepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPpsbAAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW002-PpsbA-mNeonGreen
Plasmid#205017PurposeBroad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)DepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPpsbAAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MAT2A
Plasmid#100521PurposeDox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)DepositorInsertMAT2A (MAT2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpTight TREAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE-hygro-GFP-Crumbs3a WT
Plasmid#195217PurposeRetroviral Tet response vector containing GFP tagged Crumbs3aDepositorInsertCRB3A (CRB3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
Plasmid#65662PurposeFLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A
Plasmid#65660PurposeFLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged glutamic acid 97 to alanine, lysine 100 t…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-tTRKRAB-red
Plasmid#12250DepositorInsertTetR-KRAB fusion and dsRED
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationdsRed added to backbone.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132D-RZ-Lb
Plasmid#134348PurposeGolden Gate entry vector; empty vector to clone the 2nd LbCas12a crRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131C-RZ-Lb
Plasmid#134347PurposeGolden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mTurquoise2
Plasmid#111254PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasmDepositorInsertTorA(sp)-mTurquoise2
UseTagsExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRU1064
Plasmid#14472DepositorInsertGfp-UV-GusA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRU1156
Plasmid#14473DepositorInsertGfp-mut3.1-GusA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENTR_TiTRE
Plasmid#172128PurposeEntry vector for tightly controlled gene expression by a tetracycline-responsive promoterDepositorTypeEmpty backboneUseEntry vectorTagsExpressionMammalianMutationPromotertight TREAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-TLoop-DsRedx
Plasmid#80162PurposeExpression in mammalian cellsDepositorInsertDsRedX
UseLentiviralTagsExpressionMutationPromoterCMV TetOx7Available sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mNeonGreen
Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorT(sp)-mNeonGreen
Plasmid#205021PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorT-mNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pKI1.1
Plasmid#85807PurposeCRISPR/Cas9 for Arabidopsis in pENTR/D-TOPODepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionMutationPromoterAtRPS5AAvailable sinceMay 18, 2017AvailabilityAcademic Institutions and Nonprofits only
