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We narrowed to 7,363 results for: Ank

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Showing: 7201 - 7220 of 7363 results

  1. pGL3-noSV40-humanEC1.45_p300peak1-2_3XCoordinatorMutant#5-6-7

    Plasmid
    #173993
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #5, #6 and #7 mutated
    Use
    Luciferase
    Mutation
    3X Coordinator motif mutation #5-6-7
    Promoter
    SV40 promoter
    Available Since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173993-plasmid-map-sequence-id-341706

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  5. pNB1-LOX

    Plasmid
    #154058
    Purpose
    Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    LoxP-flanked GUS and TtNAM-B1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    ZmUbi
    Available Since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154058-plasmid-map-sequence-id-313109

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  6. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  11. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  12. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

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  14. pBFC1207

    Plasmid
    #186694
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186694-plasmid-map-sequence-id-371385

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  16. pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

    Plasmid
    #177868
    Purpose
    Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d
    Insert
    mGreenLantern-based EXSISERS
    Use
    CRISPR, Synthetic Biology, and Unspecified
    Tags
    OLLAS
    Expression
    Mammalian
    Available Since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    177868-plasmid-map-sequence-id-358838

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  17. G protein alpha-s-mCherry

    Plasmid
    #66968
    Purpose
    G protein alpha-s internally tagged with mCherry and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha- s…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66968-plasmid-map-sequence-id-121428

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  20. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid
    #156415
    Purpose
    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4A-G12D (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mTagBFP2
    Mutation
    mutant KRAS4A-G12D
    Promoter
    kdrl
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156415-plasmid-map-sequence-id-308711

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Showing: 7201 - 7220 of 7363 results