We narrowed to 18,339 results for: Mut

1. Myc-Idax DBM

   Plasmid

   #68554

   Purpose

   This plasmid expresses full length IDAX containing a DNA binding mutation.

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   IDAX-DBM (Cxxc4 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T162A, H164A and Q165A mutations

   Promoter

   EF1a

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-EGFP-Cx43ML-N1

   Plasmid

   #49860

   Purpose

   Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   All internal Methionines mutated to Leucine. No s…

   Promoter

   CMV

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pEF5/FRT/DEST-3xHA/mGli2 P5-6D

   Plasmid

   #51254

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 923,956 mutated to Asp

   Promoter

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

4. H14-MBP-SUMO-Cys-Linker-BAF_L58R

   Plasmid

   #101780

   Purpose

   Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Tags

   His14-MBP-SUMO

   Expression

   Bacterial

   Mutation

   L58R mutation

   Promoter

   T5

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-WNK1__PRMT8

   Plasmid

   #116845

   Purpose

   Lentiviral expression of WNK1__PRMT8 fusion

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   WNK1__PRMT8
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

   Plasmid

   #119312

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 1-500

   Promoter

   CMV

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)

   Plasmid

   #119311

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 1-376

   Promoter

   CMV

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

   Plasmid

   #119316

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 481-852

   Promoter

   CMV

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAdEasy-EGFP-CLASP2 340-1084 9xS/A

   Plasmid

   #24525

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1084) 9xS/A (CLASP2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. B-eSpCas9

    Plasmid

    #126761

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE3320

    Plasmid

    #107529

    Purpose

    Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hLbCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

    Plasmid

    #68346

    Purpose

    Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

    Inserts

    3xgRNA;PTENA, p53B,SMAD4A
    FlPO
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. B-evoSpCas9

    Plasmid

    #126765

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1Xpress-BcrGAP R1090A

    Plasmid

    #38176

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    BCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)
    

    Use

    Tags

    Xpress

    Expression

    Mammalian

    Mutation

    aa 1004-1271 with R1090A point mutation

    Promoter

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pTAMAHISTEV_PfeAR480A-Q482A

    Plasmid

    #128946

    Purpose

    expresses R480A Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-CDC42BPB__RGS6

    Plasmid

    #116810

    Purpose

    Lentiviral expression of CDC42BPB__RGS6 fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CDC42BPB__RGS6
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-CDC42BPB__SYNJ2BP

    Plasmid

    #116811

    Purpose

    Lentiviral expression of CDC42BPB__SYNJ2BP fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CDC42BPB__SYNJ2BP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pShuttle-EGFP-CLASP2 340-1084 9xS/A

    Plasmid

    #24384

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1084) 9xS/A (CLASP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable CLASP2 deletion mutant. M…

    Promoter

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. hnRNPA2_LC_P298L_S285C

    Plasmid

    #104469

    Purpose

    express His tagged P298L S285C hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    P298L S285C

    Promoter

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-TAOK1__PIPOX

    Plasmid

    #116839

    Purpose

    Lentiviral expression of TAOK1__PIPOX fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    TAOK1__PIPOX
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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