We narrowed to 49,219 results for: REP

1. pcDNA3-mRuby2-MACROD1_d77

   Plasmid

   #172588

   Purpose

   For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD1 (MACROD1 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-76

   Promoter

   CMV

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNAU6

   Plasmid

   #135963

   Purpose

   Contains tracrRNA-hU6 sequence for double sgRNA cloning

   Depositor

   Yongming Wang

   Article

   Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175506

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CamK2-tTA

   Plasmid

   #210731

   Purpose

   AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)

   Depositor

   Wei Xu

   Article

   Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   tetracycline-controlled transactivator (tTA)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CamK2

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-6020nes

   Plasmid

   #108656

   Purpose

   A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-JNK-EV
   

   Use

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDendra2-H2b

   Plasmid

   #157817

   Purpose

   Fluorescent reporter for histone H2B tracking

   Depositor

   Xin Chen

   Article

   Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

   Insert

   Histone H2B (H2bc3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSM_ser-2prom3p::myr-mCherry

   Plasmid

   #164223

   Purpose

   Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

   Depositor

   Yan Zou

   Article

   Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

   Insert

   myr-mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   ser-2promo3

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW-ZipACR-EYFP

   Plasmid

   #88844

   Purpose

   Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   ZipACR-NotI-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5e irg1

   Plasmid

   #188698

   Purpose

   Gateway promoter element for the zebrafish irg1 gene

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   irg1 (acod1 Zebrafish)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   irg1

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB1alpha2_SulR

    Plasmid

    #186425

    Purpose

    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF287_EFS-TiCas9-P2A-Puro

    Plasmid

    #211646

    Purpose

    EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Tamoxifen-inducible SpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT024

    Plasmid

    #171636

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BFP
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSI-check2-Tab1-3'UTR

    Plasmid

    #97155

    Purpose

    For dual luciferase report assay

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    Tab1 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDendra2-H2a

    Plasmid

    #157816

    Purpose

    Fluorescent reporter for histone H2A tracking

    Depositor

    Xin Chen

    Article

    Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

    Insert

    H2A (H2ac13 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. OmEF1aCas9P2ApuroSB

    Plasmid

    #165484

    Purpose

    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

    Depositor

    Dietmar Kueltz

    Article

    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

    Insert

    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mutation

    Promoter

    Oreochromis mossambicus EF1 alpha promoter (OmEF1…

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAC_C_LMTK2

    Plasmid

    #187752

    Purpose

    MAC-tagged gene expression of human LMTK2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    LMTK2 (LMTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHBS838 TDP43 RRM-GFP

    Plasmid

    #98249

    Purpose

    TDP43 RRM-GFP reporter construct

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Internal GFP to replace RRM domains

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CRY2-CreN

    Plasmid

    #75268

    Purpose

    The second half of the photo-activatable cre recombinase

    Depositor

    Michael Bruchas

    Article

    Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

    Insert

    second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR221-SMAD3

    Plasmid

    #79507

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet-1_VNp15-DARPinOFF7

    Plasmid

    #182399

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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