We narrowed to 19,051 results for: Tec;

1. pC011 LwCas13a locus with targeting spacer

   Plasmid

   #91903

   Purpose

   LwCas13a locus into pACYC184 with targeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a with targeting spacer
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAAV-CaMKII-iGECI

   Plasmid

   #160423

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

   Plasmid

   #184952

   Purpose

   Donor for both PiggyBac and Cp36 Recombinase

   Depositor

   Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   PuroR, P2A, mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA008

   Plasmid

   #216003

   Purpose

   Fragmid fragment: (Cas protein) preferred over wildtype Cas12a

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas12a_v1.1 [EnAs]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC009 LshCas13a locus with targeting spacer

   Plasmid

   #91900

   Purpose

   LshCas13a locus into pACYC184 with targeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LshCas13a locus with targeting spacer
   

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCMV-ADARcd-YTHmut

   Plasmid

   #194431

   Purpose

   ADAR1 catalytic domain E488Q fused to YTHmut

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   ADARcd fused to YTHmut domain (ADAR Human)
   

   Tags

   ADARcd-YTHmut-HA

   Expression

   Mammalian

   Mutation

   m6A binding region of YTH domain is deleted

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas12f-GE ver4.0

   Plasmid

   #176543

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.0
   Cas12f-GE ver4.0
   

   Expression

   Mammalian

   Promoter

   U6 and chicken β-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CABE-T2.19

   Plasmid

   #193265

   Purpose

   Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T2.19
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-HA-Flag-natT1R2

   Plasmid

   #113945

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Promoter

   CMV

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBJ 2358

    Plasmid

    #89245

    Purpose

    Expresses YFP fused to the N-terminus of Mouse Capping Protein Alpha1, using the Clontech vector PEYFP C-1.

    Depositor

    John Cooper

    Article

    Edwards et al Nat Commun. 2015 Sep 28;6:8415. doi: 10.

    Insert

    Capza1 (Capza1 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid

    #181789

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. YE1-BE4max-CP1028

    Plasmid

    #138160

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAWH-largeT

    Plasmid

    #133895

    Purpose

    Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    LargeT
    

    Expression

    Yeast

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpCas9 TadCBEc

    Plasmid

    #193834

    Purpose

    Express TadCBEc (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDc-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT/TO-N-mEGFP-PARP6

    Plasmid

    #178005

    Purpose

    Expresses N-terminally mEGFP-tagged PARP6

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP6 (PARP6 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT/TO-N-mEGFP-PARP15

    Plasmid

    #178000

    Purpose

    Expresses N-terminally mEGFP-tagged PARP15

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP15 (PARP15 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-LMP2A-IRES-GFP

    Plasmid

    #220352

    Purpose

    Expresses EBV latent gene LMP2A in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b LFn-Fla 3A

    Plasmid

    #84872

    Purpose

    Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Fla
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    3 C-terminal leucines have been replaced by alani…

    Promoter

    T7

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

19. mEos2-Actin-7

    Plasmid

    #57339

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Actin (ACTB Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. Cerulean-GalT

    Plasmid

    #11930

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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