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KTK_042

Plasmid

#180530

Purpose

Entry vector containing J23100 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23100 anderson promoter

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_dead_mt

Plasmid

#167270

Purpose

Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Mutation

C145A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast_dead_mt

Plasmid

#167272

Purpose

Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R-C145A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTone-gata2aECE-EGFP

Plasmid

#132974

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

eGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2001

Plasmid

#105871

Purpose

High copy HRPT backbone plasmid for insertion of MiniP promoters

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Type

Empty backbone

Available Since

Feb. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMT3-RhoGC D380E

Plasmid

#100035

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC D380E

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

D380E

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC D380N

Plasmid

#100034

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC D380N

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

D380N

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMElmo2P1gfp

Plasmid

#90127

Purpose

Gateway Mid-entry clone containing promoter sequence specific for lmo2 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMEdusp5P1gfp

Plasmid

#90126

Purpose

Gateway Mid-entry clone containing promoter sequence specific for dusp5 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMEdll4P1gfp

Plasmid

#90125

Purpose

Gateway Mid-entry clone containing promoter sequence specific for dll4 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4P1Egfp (dll4 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1962

Plasmid

#82572

Purpose

High copy number plasmid containing Ple303 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple303

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1963

Plasmid

#82573

Purpose

High copy number plasmid containing Ple304 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple304

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1961

Plasmid

#82571

Purpose

High copy number plasmid containing Ple302 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple302

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1960

Plasmid

#82570

Purpose

High copy number plasmid containing Ple301 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple301

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1957

Plasmid

#82568

Purpose

High copy number plasmid containing hs1218 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

hshs1218

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1953

Plasmid

#82567

Purpose

High copy number plasmid containing hs671 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

hs671

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1944

Plasmid

#82566

Purpose

High copy plasmid containing Ple267 (UGT8 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple267

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pDECKO-mCherry MALAT1_Enhancer.4

Plasmid

#78539

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Enhancer.3

Plasmid

#78538

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.3

Plasmid

#78542

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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