We narrowed to 45,157 results for: REP

1. pCAGGS_m-AKAR5’

   Plasmid

   #114140

   Purpose

   Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   AKAR5' C-terminally tagged by KRas C tail
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5FRT/TO-UBE2QL1-GFP

   Plasmid

   #124667

   Purpose

   Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. R725-X09-635: His6-tev-Hs.SPRED1(13-125)

   Plasmid

   #159581

   Purpose

   Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

   Depositor

   Dominic Esposito

   Article

   Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

   Insert

   His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1371-sgITGB1-3

   Plasmid

   #121534

   Purpose

   sgITGB1-3 sequence: GTTCAGTGAATGGGAACAACG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

   Insert

   sgITGB1-3
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSN67

   Plasmid

   #100124

   Purpose

   expresses putative KLF4 DBD with a 6xHis tag

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Insert corresponds to the DBD of KLF4

   Promoter

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. NG1301 pCI-TPI-4MS2-SMG5(short)-4H

   Plasmid

   #65806

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Use

   Tags

   4 MS2 stem loops, SMG5 3'UTR shortened versi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2-Cx43-NoStart

   Plasmid

   #49853

   Purpose

   Encodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Start codon mutated to from methionine to isoleuc…

   Promoter

   CMV

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

8. FgH1tUTG_huMcl-1.2

   Plasmid

   #85531

   Purpose

   Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   hu Mcl-1.2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1t

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-Hygro-TRE>hHES3:T2A:EGFP

   Plasmid

   #236630

   Purpose

   Hygromycin selected lentiviral construct with tetracyclin-inducible human HES3

   Depositor

   Genevieve Kendall

   Article

   Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

   Inserts

   HES3 (HES3 Human)
   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized based on a variant of wildtype GF…

   Promoter

   TRE

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXJ-His-CD274

    Plasmid

    #232659

    Purpose

    transient expresses PD-L1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLAS2w.Phyg-CHST8

    Plasmid

    #232650

    Purpose

    expresses CHST8 wild-type in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CHST8 (CHST8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXJ-His-CD86

    Plasmid

    #232666

    Purpose

    transient expresses CD86 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ-His-CD80

    Plasmid

    #232665

    Purpose

    transient expresses CD80 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD80 (CD80 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKT2/CAGXSP/GFPCD63

    Plasmid

    #231709

    Purpose

    Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    gfp

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB9v2

    Plasmid

    #210029

    Purpose

    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB1163

    Plasmid

    #210032

    Purpose

    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB758

    Plasmid

    #210033

    Purpose

    Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas7-P2A-Cas5-T2A-Cas8
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB9

    Plasmid

    #210036

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    puro-T2A-eGFP
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Tom20-ECFP-FRB

    Plasmid

    #210289

    Purpose

    mitochondrial OM marker and "anchor" in FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    Tom20-ECFP-FRB
    

    Use

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ECFP-FRB-Sec61b

    Plasmid

    #210290

    Purpose

    nucleus OM marker and "anchor" for FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    ECFP-FRB-Sec61b
    

    Use

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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