161,398 results

1. 1B-GFP1-10

   Plasmid

   #193867

   Purpose

   E. coli expression vector for Hisx6 tagged GFP1-10.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   sfGFP1-10
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTECH-MmPylRS(IPYE)

   Plasmid

   #104073

   Purpose

   Expression plasmid encoding MmPylRS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MmPylRS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pax5

   Plasmid

   #35003

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Paired box protein Pax-5 (Pax5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-mEGFP-SQSTM1-WT

   Plasmid

   #194582

   Purpose

   Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcVpx.myc

   Plasmid

   #213388

   Purpose

   Generate lentiviral vector which increases gene delivery into primary human myeloid cells

   Depositor

   Nathaniel Landau

   Article

   Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

   Insert

   SIVmac vpx (vpx Simian Immunodeficiency Virus)
   

   Use

   Lentiviral

   Tags

   myc-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas12aUltra_5XNLS

   Plasmid

   #218775

   Purpose

   His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

   Depositor

   Ross Wilson

   Article

   Sahu et al bioRxiv 2024.07.14.603391

   Insert

   5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
   

   Use

   CRISPR

   Tags

   6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

   Expression

   Bacterial

   Mutation

   Nucleotides mutations were made to encode for arg…

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AP1muA-TurboID-polyA-G418 HR

   Plasmid

   #227734

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   Turbo-ID, V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK054 pDEST exorh:mCerulean

   Plasmid

   #195985

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-mStayGold-Puro

   Plasmid

   #227253

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep

    #154868-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ulrik Gether

    Promoter

    Human Synapsin-1

    Tags

    mCherry (Flp-dependent)

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pCK306

    Plasmid

    #110544

    Purpose

    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    PrhaBAD
    yfp
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A, PrhaBAD, and kanR promoter (upstream of kanR…

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-AHPH-DM

    Plasmid

    #71368

    Purpose

    Location sensor for RhoA-GTP

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    C terminus of Anillin (ANLN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A740D and E758K

    Promoter

    CMV

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC2A3

    Plasmid

    #132114

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A3 (SLC2A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pIRES-HMGA1

    Plasmid

    #13466

    Depositor

    Edward Whang

    Article

    Liau et al Cancer Res. 2006 Dec 15. 66(24):11613-22

    Insert

    HMGA1 (HMGA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pTP284

    Plasmid

    #113312

    Purpose

    human Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

    Insert

    Nbs1 (NBN Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4-FLAG-Jhdm2a

    Plasmid

    #38136

    Depositor

    Toru Nakano, Toshinobu Nakamura

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b-His-RF2

    Plasmid

    #124130

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Release factor 2 (prfB Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. V5-LOV-Turbo-NES

    Plasmid

    #199545

    Purpose

    expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NES and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-RSV-tdTomato-2A-GCaMP6s

    Plasmid

    #80316

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Darrell Kotton

    Article

    Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

    Inserts

    tdTomato
    GCaMP6s
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    RSV

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6-V5-Topo mCherry

    Plasmid

    #128062

    Purpose

    Lentiviral vector for C terminal mCherry fusions

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge