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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Publication

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Halo-TOMM20-N-10

Plasmid

#123284

Purpose

Localization: Outer Mitochondrial Membrane

Depositor

Kevin McGowan

Insert

TOMM20 (TOMM20 Human)

Tags

HaloTag7

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHAGE-PIK3CA

Plasmid

#116771

Purpose

Lentiviral expression of PIK3CA

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

Plasmid

#121966

Purpose

Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

scFv-GCN4

Use

Lentiviral

Tags

superfold GFP-GB1-NLS-iLID

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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SP-GFP-CD274-NU

Plasmid

#121139

Purpose

Mammalian expression of GFP tagged CD274

Depositor

Christine Mayr

Publication

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Inserts

CD274 (CD274 Human)
CD274 (CD274 Human)

Expression

Mammalian

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

Plasmid

#115211

Purpose

Expresses PsmCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PsmCas13

Tags

SUMO

Expression

Bacterial

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Publication

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDawn-sfGFP

Plasmid

#107741

Purpose

Expresses superfolder GFP under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Publication

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

superfolder GFP

Expression

Bacterial

Promoter

pLambda

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET246-pUC57 12xMS2V6

Plasmid

#104390

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Publication

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAVS1-idCas9-vpr

Plasmid

#89985

Purpose

inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

Depositor

Jie Na

Publication

Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

Insert

dCas9-VPR

Expression

Mammalian

Promoter

TRE-tight

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCold-I-Dsup

Plasmid

#90021

Purpose

To express His-tagged Dsup in bacteria

Depositor

Takekazu Kunieda

Publication

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

His

Expression

Bacterial

Promoter

cspA

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

Pooled library

#1000000095

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Eric Lander, David Sabatini

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

Pooled library

#83976

Purpose

Human CRISPRi Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Publication

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only
pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Publication

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pK031.TRE-Cre (Supernova)

Plasmid

#69136

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Publication

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

NLS-Cre

Expression

Mammalian

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Publication

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCIneo RYR-1

Plasmid

#75113

Purpose

Clone encoding rabbit ryanodine receptor in mammalian expression vector

Depositor

Paul Allen, Robert Dirksen

Publication

Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

Insert

Ryanodine receptor 1

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Publication

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGEx-4T-1-YTHDF3

Plasmid

#70088

Purpose

Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles