Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,415 results for: ESP

Showing: 7261 - 7280 of 7415 results

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pCS2+8-CYP2A6(C82A)-flag-tev-halo-his

Plasmid

#227160

Purpose

For T-REX experiments of CYP2A6 C82A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C439A)-flag-tev-halo-his

Plasmid

#227161

Purpose

For T-REX experiments of CYP2A6 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA HCAR3

Plasmid

#226811

Purpose

Tet-inducible lentiviral vector for 2xHA HCAR3 expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

HCAR3 (HCAR3 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_006009.2

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP35

Plasmid

#196773

Purpose

Contains RCD1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

RADICAL-INDUCED CELL DEATH1 (RCD1 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

248 G->C

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP07

Plasmid

#196745

Purpose

Contains CERK1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

CHITIN ELICITOR RECEPTOR KINASE 1 (CERK1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

Plasmid

#195018

Purpose

An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

Depositor

Christopher Richie

Article

Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

Inserts

CCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG

Use

AAV

Tags

KASH domain of Nesprin2

Promoter

EF1a, hU6, and mU6

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Mpro protease (T190I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204786

Purpose

Bacterial expression of Sars Cov 2 MPRO T190I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

T190I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (Q189K mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204784

Purpose

Bacterial expression of Sars Cov 2 MPRO Q189K mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

Q189K

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (P132H mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204783

Purpose

Bacterial expression of Sars Cov 2 MPRO P132H mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

P132H

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (N142S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204782

Purpose

Bacterial expression of Sars Cov 2 MPRO N142S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

N142S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204779

Purpose

Bacterial expression of Sars Cov 2 MPRO M49T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204777

Purpose

Bacterial expression of Sars Cov 2 MPRO M49I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (G143S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204776

Purpose

Bacterial expression of Sars Cov 2 MPRO G143S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

G143S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A191V mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204774

Purpose

Bacterial expression of Sars Cov 2 MPRO A191V mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A191V

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A193T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204773

Purpose

Bacterial expression of Sars Cov 2 MPRO A193T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A193T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A191T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204772

Purpose

Bacterial expression of Sars Cov 2 MPRO A191T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A191T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (A193S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204771

Purpose

Bacterial expression of Sars Cov 2 MPRO A193S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A193S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

Plasmid

#204769

Purpose

Bacterial expression of Sars Cov 2 MPRO C145S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

C145S mutant

Available Since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUGP1.1

Plasmid

#196615

Purpose

Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRB133.2

Plasmid

#181950

Purpose

aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)

Tags

mNeonGreen

Expression

Bacterial

Promoter

PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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