We narrowed to 16,198 results for: grna

1. PB-GG-OCT4-1-5-PGK-Puro

   Plasmid

   #102893

   Purpose

   PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

   Plasmid

   #91881

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Suz12 (Suz12 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pROS13

   Plasmid

   #107927

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

   Plasmid

   #91882

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Suz12 (Suz12 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

5. LADL Bridge + Promoter Only Target

   Plasmid

   #127669

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a and hU6

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. OA-1045D

   Plasmid

   #125006

   Purpose

   Expresses gRNAs targeting hedgehog and wingless

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   wg gRNA (wg Fly)
   

   Expression

   Insect

   Promoter

   u6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

   Plasmid

   #217342

   Purpose

   crRNA array targeting CD81, B2M, KIT, CD55

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
   crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
   crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
   crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
   crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
   crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-EEA-5g-OSK2M2L1-PGK-Puro

   Plasmid

   #102909

   Purpose

   PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. EasyClone-MarkerFree Vector Set

   Plasmid Kit

   #1000000098

   Purpose

   Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9

   Depositor

   Irina Borodina

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pHnS 2comp Donor;smFP-V5 KO;Template ORF0

    Plasmid

    #240304

    Purpose

    Donor:smFP-V5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    Template gRNA
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTRY4_ Pi21

    Plasmid

    #126904

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1197R_gZBF-ER

    Plasmid

    #241825

    Purpose

    gRNA expressing plasmid with broken SEPARATOR

    Depositor

    Omar Akbari

    Insert

    Actin5C promoter
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHnS 2comp Donor;smFP-V5 KO;Template ORF2

    Plasmid

    #240306

    Purpose

    Donor:smFP-V5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    Template gRNA
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHnS 2comp Donor;smFP-V5 KO;Template ORF1

    Plasmid

    #240305

    Purpose

    Donor:smFP-V5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    Template gRNA
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHnS KI;Actb Donor;3xV5 KO;Template

    Plasmid

    #240291

    Purpose

    KI:Actb Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Actb
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHnS KI;Sptbn4 Donor;3xV5 KO;Template

    Plasmid

    #240295

    Purpose

    KI:Sptbn4 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Sptbn4
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHnS KI;Gria1 Donor;3xV5 KO;Template

    Plasmid

    #240293

    Purpose

    KI:Gria1 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Gria1
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHnS KI;Pdha1 Donor;3xV5 KO;Template

    Plasmid

    #240300

    Purpose

    KI:Pdha1 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Pdha1
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHnS KI;Lmnb1 Donor;3xV5 KO;Template

    Plasmid

    #240297

    Purpose

    KI:Lmnb1 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Lmnnb1
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UC16m

    Plasmid

    #121038

    Purpose

    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    gQi Cas9 gRNA UC part
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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