We narrowed to 59,591 results for: ran

1. 719-2µ-minHIS3

   Plasmid

   #40610

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   HIS3 (HIS3 Budding Yeast, Synthetic)
   

   Tags

   HA

   Expression

   Yeast

   Mutation

   Every codon of the coding sequence has been repla…

   Promoter

   TDH3

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK520

   Plasmid

   #155386

   Purpose

   PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   PphlF-TA1 in dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSGV-1D3-28Z All ITAMs intact

   Plasmid

   #107226

   Purpose

   Anti-mouse CD19 CAR with CD3 ITAMs intact

   Depositor

   James Kochenderfer, Steven Rosenberg

   Article

   Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

   Insert

   1D3-28Z All ITAMs intact
   

   Use

   Retroviral

   Tags

   None

   Mutation

   None

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-FLAG-C12orf66

   Plasmid

   #87047

   Purpose

   mammalian expression of C12orf66

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   C12orf66 (C12orf66 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

   Plasmid

   #247160

   Purpose

   Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   Puromycin resistance, mCherry
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTmpl-miniDonor-GFP

   Plasmid

   #238107

   Purpose

   Mini donor expressing GFP, used for PCR to generate IVT template.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence
   

   Expression

   Mammalian

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. opto-Cdc42

   Plasmid

   #174510

   Purpose

   Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Cdc42-mCherry
   

   Tags

   3xFLAG and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG/ATP8B3-HA

   Plasmid

   #209211

   Purpose

   Mammalian expression of ATP8B3

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP8B3 (ATP8B3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/mStayGold(c4)=UtrCH

   Plasmid

   #212020

   Purpose

   For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   UtrCH
   

   Tags

   mStayGold(c4)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 deltaN89 Beta-catenin

    Plasmid

    #19288

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-89

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pX330-SpCas9-NG

    Plasmid

    #117919

    Purpose

    Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-CAG::Nucbow

    Plasmid

    #158992

    Purpose

    Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Nucbow
    

    Tags

    All FPs are fused to H2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL2-p21 promoter-Luc

    Plasmid

    #33021

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    p21 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    p21

    Available Since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9_T2A_mCherry sgBFP

    Plasmid

    #192929

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLDLR-Luc (aka: pES7)

    Plasmid

    #14940

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2-MT GLI2 delta N

    Plasmid

    #17649

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Missing 328 N-terminal amino acids

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHTNW

    Plasmid

    #136403

    Purpose

    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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