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pUASTattb AaegOr4-B

Plasmid

#59793

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele B

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele B

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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MIGR1-SV40-hygro

Plasmid

#254096

Purpose

Retroviral transfer empty vector, hygromycin selection marker.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Type

Empty backbone

Use

Retroviral

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(deltaA)B

Plasmid

#248938

Purpose

Hachiman from P. fluorescens under pTet control with the dHamA domain removed

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamMA(deltaA)B

Expression

Bacterial

Mutation

HamA domain deletion

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDL1-eGFP

Plasmid

#242945

Purpose

Encodes a fragment of eGFP for generating dsRNA for use as a control in RNAi experiments

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

eGFP

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3399

Plasmid

#234083

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

DasherGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3225

Plasmid

#234087

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

DasherGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-DR

Plasmid

#234800

Purpose

Mammalian expression of bovine arrestin-3 (D291R)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

D291R

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-RE

Plasmid

#234799

Purpose

Mammalian expression of bovine arrestin-3 (R170E)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

R170E

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3194

Plasmid

#234094

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

DasherGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3231

Plasmid

#234103

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

DasherGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMBS-844

Plasmid

#217998

Purpose

Nucleoplasmin NLS for protein targeting to nucleus

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Nucleoplasmin NLS

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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EC0_6_sgRNA

Plasmid

#163717

Purpose

Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

sgRNA expression cassette

Use

Synthetic Biology; Moclo vector level 0 (type 234)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pKP476

Plasmid

#167594

Purpose

Express TonB ∆(199-216)

Depositor

Kathleen Postle

Article

Postle et al bioRxiv 2021.12.08.471779

Insert

tonB ∆(199-216)

Expression

Bacterial

Mutation

tonB ∆(199-216)

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJK_proB_amiE

Plasmid

#59836

Purpose

Constitutively expresses AmiE with proB promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proB

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK1_amiE

Plasmid

#59843

Purpose

Constitutively expresses AmiE with proK1 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK16_amiE

Plasmid

#59838

Purpose

Constitutively expresses AmiE with proK16 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK16

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK14_amiE

Plasmid

#59839

Purpose

Constitutively expresses AmiE with proK14 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK14

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK9_amiE

Plasmid

#59840

Purpose

Constitutively expresses AmiE with proK9 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK9

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK6_amiE

Plasmid

#59841

Purpose

Constitutively expresses AmiE with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK6

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK3_amiE

Plasmid

#59842

Purpose

Constitutively expresses AmiE with proK3 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK3

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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