We narrowed to 15,822 results for: LEA

1. pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V)

   Plasmid

   #154875

   Purpose

   Expresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfBAEBL (PF3D7_0506900 P. falciparum)
   

   Use

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   I1134V, P1135L, Y1136V, F1137V; insert codes for …

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)

   Plasmid

   #154977

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   P1424/Y1425I/Y1426I; insert codes for EBA175 resi…

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLCHKO_PDPR_exon_deletion_2

   Plasmid

   #155066

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLCHKO_SFRS7_exon_deletion_1

   Plasmid

   #155069

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLCHKO_SFRS7_exon_deletion_2

   Plasmid

   #155070

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLCHKO_MDM4_exon_deletion_1

   Plasmid

   #155073

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLCHKO_MDM4_exon_deletion_2

   Plasmid

   #155074

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLCHKO_MDM4_exon_deletion_4

   Plasmid

   #155076

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLCHKO_Ptbp_ex8_1

   Plasmid

   #155081

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLCHKO_Ptbp_ex8_4

    Plasmid

    #155084

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLCHKO_SFRS7_exon_deletion_4

    Plasmid

    #155072

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLCHKO_Ptbp_ex8_2

    Plasmid

    #155082

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLCHKO_PDPR_exon_deletion_3

    Plasmid

    #155067

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCHKO_PDPR_exon_deletion_4

    Plasmid

    #155068

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FUG-T2A-F241S

    Plasmid

    #140740

    Purpose

    Lentiviral vector to overexpress the autism-associated PTEN mutation F241S and GFP as two separate proteins

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-T2A-PTENF241S (PTEN Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    F241S

    Promoter

    hUbiC

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUG-T2A-W274L

    Plasmid

    #140742

    Purpose

    Lentiviral vector to overexpress the autism-associated PTEN mutation W274L and GFP as two separate proteins

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-T2A-PTENW274L (PTEN Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    W274L

    Promoter

    hUbiC

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUG-T2A-D252G

    Plasmid

    #140741

    Purpose

    Lentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteins

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-T2A-PTEND252G (PTEN Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    D252G

    Promoter

    hUbiC

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBB0405-TEV-His12

    Plasmid

    #137040

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRevA-TEV-His12

    Plasmid

    #137034

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pP13-TEV-His12

    Plasmid

    #137035

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge