We narrowed to 166,390 results for: addgene

1. pT2-cryR;dll4CE2-P1Egfp

   Plasmid

   #90147

   Purpose

   contains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   dll4E2P1Egfp (dll4 Zebrafish)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_TT S6K (E389-deltaCT)

   Plasmid

   #58507

   Purpose

   Gateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCT

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   Rps6kb1 (Rps6kb1 Rat)
   

   Use

   Entry vector

   Tags

   HA

   Mutation

   T389E, stop codon at amino acid 402

   Available Since

   Aug. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

3. MAC_N_RAB11A

   Plasmid

   #111691

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   RAB11A (RAB11A Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEG R2-iNeo-L3 #BGV207

   Plasmid

   #48986

   Purpose

   Supplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   neomycin resistance
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Promoter

   NA

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. LIPI_pCSdest

   Plasmid

   #53849

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   LIPI (LIPI Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

   Plasmid

   #170817

   Purpose

   Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mNeonGreen-P2A-BlastR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. KCNJ15_pCSdest

   Plasmid

   #53840

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   KCNJ15 (KCNJ15 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pU6-(BsaI)_CBh-Cas9

   Plasmid

   #135010

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Tags

   3xFLAG (N terminal on insert)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FHp5pUP95C3,5SGW (E5)

   Plasmid

   #74035

   Purpose

   lentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Mutation

   PCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…

   Promoter

   H1

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

10. gh172

    Plasmid

    #106833

    Purpose

    expression of gRNA targeting UGGT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGGT1 (UGGT1 Human)
    

    Expression

    Mammalian

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5xtetO (tet3) Gene Desert

    Plasmid

    #131339

    Purpose

    gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Desert-gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIS1 IMP-1 long-mut-let-7

    Plasmid

    #21642

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant let-7 microRNA binding sites

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. gh121

    Plasmid

    #106780

    Purpose

    expression of gRNA targeting ST8SIA1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST8SIA1 (ST8SIA1 Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJS278

    Plasmid

    #134139

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Expression

    Bacterial

    Mutation

    aa 132-255, R155A

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.2 mir-1-1 reporter hsa-mir-138-2

    Plasmid

    #46677

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-138-2 (MIR138-2 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEG R2-eCFP-L3 #BGV202

    Plasmid

    #48977

    Purpose

    Supplies the selection marker (eCFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    eCFP
    

    Use

    Gateway cloning vector

    Tags

    IRES (strong)

    Promoter

    NA

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. FB001

    Plasmid

    #119057

    Purpose

    Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter trpC
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

    Plasmid

    #134334

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA17_Sga
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

    Plasmid

    #134335

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA18_Sma
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

    Plasmid

    #134336

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA19_Ssim
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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