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cASIC1 concatemer - Subunit B

Plasmid

#233130

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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cASIC1 concatemer - Subunit A

Plasmid

#233129

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α-loxP-GCaMP6f-loxP-WPRE

Plasmid

#233288

Purpose

AAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.

Depositor

Dayu Lin

Article

Fang et al Neuron. 2018 Apr 4;98(1):192-207.e10. do

Insert

GCaMP6f

Use

AAV

Promoter

EF1α

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pJH4040

Plasmid

#229803

Purpose

Pgpc-1 wCherry unc-54 3' UTR C.elegans neuron and other structures expression of RFP

Depositor

Mei Zhen

Article

Lin et al Sci Adv. 2023 Mar;9(9):eade1249. doi: 10

Insert

Pgpc-1 wCherry unc-54 3' UTR

Tags

RFP

Expression

Worm

Promoter

Pgpc-1

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLVUT-eC3GFP

Plasmid

#225485

Purpose

Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Exoenzyme C3 transferase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 30-251, See Depositor Comments

Promoter

Human ubiquitin C

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLVUT-spGFP

Plasmid

#225486

Purpose

Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Igκ leader and TAT peptide

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Human ubiquitin C

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-eGFP-NFATC2IP SLD2

Plasmid

#221438

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP SUMO-like domain 2 (SLD2, aa 345-418)

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

eGFP

Expression

Mammalian

Mutation

Amino acids 1-344 of NFATC2IP deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4693

Plasmid

#200784

Purpose

Ptwk-40s EGFP unc-54 3' UTR C.elegans AVA and other neurons expression of EGFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

no

Tags

GFP

Expression

Worm

Promoter

Ptwk-40s

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4506

Plasmid

#200255

Purpose

Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

TeTx

Tags

mCherry

Expression

Worm

Promoter

Pnpr-4

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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TR knockout HRD

Plasmid

#207579

Purpose

Homologous recombination donor replacing TR with a puro cassette.

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

puro resistance cassette flanked by TR locus sequences excluding TR

Tags

None

Expression

Mammalian

Promoter

SV40

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1-2

Plasmid

#203837

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1-2

Expression

Mammalian

Mutation

KO of both AP1 sites

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1

Plasmid

#203835

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Expression

Mammalian

Mutation

KO of AP1 upstream

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP2

Plasmid

#203836

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Expression

Mammalian

Mutation

KO of AP1 downstream

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF2

Plasmid

#200376

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-V5-Nxph1

Plasmid

#184336

Purpose

Expression of V5-tagged mouse neurexophilin-1

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

neurexophilin-1 (Nxph1 Mouse)

Tags

V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xHA-Syn-smFP-flag

Plasmid

#182563

Purpose

For mouse Nav1.6 knockin with 3xHA at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xHA donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-HaloTag-V5-EF1-sfGFP

Plasmid

#182565

Purpose

For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xV5-EF1-smFP-flag

Plasmid

#182562

Purpose

For mouse Nav1.6 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2108

Plasmid

#179252

Purpose

Punc-4::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Punc-4

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2106

Plasmid

#179250

Purpose

Pnmr-1::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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