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pUPD3_FULLPRM_Para

Plasmid

#243912

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site. Lacks activator protein, AraC.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

ParaBAD without araC

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Ptet

Plasmid

#243914

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site. Lacks repressor, TetR.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PtetA without tetR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PR

Plasmid

#243916

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site. Lacks repressor, cI857.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PR without cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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EKAREN5-c

Plasmid

#246761

Purpose

Control plasmid for EKAREN5 and EKAREN5-gl (original YPet - mTurquoise-GL pair)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-c

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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EKAREN5-y

Plasmid

#246762

Purpose

Control plasmid for EKAREN5 and EKAREN5-gl (synonymous variant YPet - mTurquoise2 pair)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-y

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF-cascade

Plasmid

#247069

Purpose

L-arabinose inducible E.coli cascade operon inserted in pCDF vector

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

E.coli Type I-E CRISPR cas

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b-Rhobin9

Plasmid

#239019

Purpose

Bacterial overexpression of de novo Rhodamine binder 9 (Rhobin2) protein

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

RhoBin9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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DriD_78-327

Plasmid

#237853

Purpose

DriD missing the wHTH DNABD used to crystallize the apo protein

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 78-327

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo

Plasmid

#221725

Purpose

Expression of Zeocin resistance marker in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-dsRed

Plasmid

#217006

Purpose

lentiviral plasmid for gRNA cloning with selectable dsRed expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP3667

Plasmid

#222353

Purpose

Expression vector with GFP expressed under PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SV40p-gI-BlastR

Plasmid

#207182

Purpose

Gateway compatible Lentiviral Expression Plasmid SV40p-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RBP-WDR5

Plasmid

#209702

Purpose

Mammalian expression of WDR5 with N terminal RBP fusion

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

RBP-WDR5

Tags

RBP

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-Gamillus(2x)

Plasmid

#197921

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus-Gamillus

Expression

Mammalian

Mutation

none

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mCardinal

Plasmid

#197258

Purpose

Expresses the protein mCardinal in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-Clover

Plasmid

#209721

Purpose

Mammalian expression of clover localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-Clover

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5

Plasmid

#209706

Purpose

Mammalian expression of y-ATOM biosensor for WDR5

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_SH2

Plasmid

#209708

Purpose

Mammalian expression of y-ATOM biosensor for SH2

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_SH2

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-SH2

Plasmid

#209717

Purpose

Mammalian expression of SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-SH2

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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