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GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7repeats)-GFP-Pho8

Plasmid

#207011

Purpose

Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

GFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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OMMGFP 1-10

Plasmid

#164119

Purpose

Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

OMMGFP 1-10

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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MT363

Plasmid

#65858

Purpose

The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

nahG

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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F-IRF7

Plasmid

#169777

Purpose

Express IRF7 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)

Tags

Flag tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Tags

His6-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSB90 - pAGM4723_VirGN54D

Plasmid

#123187

Purpose

Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Type

Empty backbone

Use

Level 2 acceptor vector for expression in plants

Expression

Plant

Mutation

VirGN54D for increase virulence

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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EF-hGPC3

Plasmid

#24670

Depositor

Jorge Filmus

Article

Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

Insert

GPC3 (GPC3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
fabp10a:H2B-mGreenLantern

Plasmid

#230045

Purpose

Expression of nuclear green fluorescent protein in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

Insert

mGreenLantern

Use

Zebrafish expression

Tags

H2B

Promoter

fabp10a

Available Since

Feb. 5, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-AncBE4max

Plasmid

#174164

Purpose

This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

Depositor

Wensheng Wei

Article

Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

Insert

AncBE4max

Use

Lentiviral

Tags

P2A-mCherry

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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SpySwitch

Plasmid

#184225

Purpose

Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpySwitch

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a-dCas9-KRAB-GFP backbone

Plasmid

#194281

Purpose

Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIVM_26

Plasmid

#204501

Purpose

Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

Depositor

Alessio Ciulli

Article

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)

Expression

Bacterial

Mutation

0

Promoter

AmpR

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLPCX mito Grx1-roGFP2

Plasmid

#64977

Purpose

Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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psicheck2-PHLPP2mut

Plasmid

#203606

Purpose

Reporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR were m…

Promoter

SV40

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB

Plasmid

#166145

Purpose

Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10

Expression

Bacterial

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

Plasmid

#164510

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244092) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Has Service

AAV9

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Mutation

Synthetic Construct

Promoter

Synapsin

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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