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pSP64-hKCNQ1

Plasmid

#53048

Purpose

Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)

Use

Xenopus oocyte expression

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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TPC2-D276K-G-GECO1.2

Plasmid

#207142

Purpose

Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

G-GECO1.2

Expression

Mammalian

Mutation

Changed Aspartate 276 to Lysine

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCR1163

Plasmid

#111099

Purpose

Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCD2 gRNA (FANCD2 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

hSyn (human synapsin)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-TBX20a

Plasmid

#98620

Purpose

Gateway entry TOPO TA cloning vector for TBX20a

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Tbx20a (Tbx20 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P2

Plasmid

#64786

Purpose

Luciferase expression driven by miR-34a promoter P2

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P2 (MIR34A Human)

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCR1164

Plasmid

#111100

Purpose

Expresses FANCD2_-_10068140.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCD2 gRNA (FANCD2 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRVdGL-1Cre

Plasmid

#98038

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRVdG-4GCaMP6f

Plasmid

#98035

Purpose

First-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf48

Plasmid

#12736

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf48

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pRVdGL-1EGFP

Plasmid

#98036

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE722

Plasmid

#103022

Purpose

expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHCAN1-4.S (CAN1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1272

Plasmid

#111107

Purpose

Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCI gRNA (FANCI Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE714

Plasmid

#103021

Purpose

expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHIS4-4.S (HIS4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf35

Plasmid

#12723

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf35 (J3R Vaccinia Virus)

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pACYC-mTET1-N

Plasmid

#81056

Purpose

bacterial expression of N-terminus of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

N-terminus, amino acids 301-1366

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCR1110

Plasmid

#111096

Purpose

Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

BLM gRNA (BLM Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1322

Plasmid

#111109

Purpose

Expresses FANCL_+_58468435.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCL gRNA (FANCL Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1271

Plasmid

#111106

Purpose

Expresses FANCI_-_89787239.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCI gRNA (FANCI Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFP59

Plasmid

#247215

Purpose

Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (tDF30ppr) from an E. coli sigma70 promoter. Suitable for cell-free expression.

Depositor

Elizabeth Strychalski

Article

Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26

Inserts

superfolder GFP
Dimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)

Use

Synthetic Biology; E. coli-based cell-free expres…

Tags

Streptavidin tag

Expression

Bacterial

Mutation

Two mutations were made to the second Pepper unit…

Promoter

Pj23100 (ttgacggctagctcagtcctaggtacagtgctagc)

Available Since

May 29, 2026

Availability

Academic Institutions and Nonprofits only

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