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PiggyBac_24mer_pegArray_bb

Plasmid

#238545

Purpose

Golden gate acceptor backbone for 24mer pegArray assemblies

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP C1

Plasmid

#202424

Purpose

Encodes GFP alone

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP5

Plasmid

#66584

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Expression

Mammalian

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pRubiC

Plasmid

#66700

Purpose

Express mCherry in Newborn neuorns

Depositor

Bryan Luikart

Article

Luikart et al PLoS One. 2011;6(5):e19077. doi: 10.1371

Insert

mCherry

Use

Retroviral

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-CACNG3

Plasmid

#140590

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CACNG3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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ADC BAC Reporter

Plasmid

#78222

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGWT35S

Plasmid

#182790

Purpose

Destination vector for transient expression in plant

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Type

Empty backbone

Expression

Plant

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Promoter

tight TRE

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-P1

Plasmid

#120863

Purpose

Encodes permuteposon P1 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P1

Use

Synthetic Biology

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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YPRCΔ15α1

Plasmid

#101712

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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TP901 GFP BAC Reporter

Plasmid

#78216

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
MB 103C ttrSR(m13)-Bxb1_P7-sfGFP_mCherry

Plasmid

#232475

Purpose

Optimized tetrathionate sensor with recombinase switch and fluorescent reporter (GFP), constitutive mCherry

Depositor

Mikhail Shapiro

Article

Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

Inserts

ttrS
ttrR
PttrB185-269
sfGFP
AxeTxe
mCherry
Bxb1 integrase

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2_EYFP_EcoRI_SV40_Recorder

Plasmid

#250552

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of an empty recorder containing an EcoRI site.

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

EYFP_EcoRI_SV40_Recorder

Use

Synthetic Biology

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pSB1K3_M13ps

Plasmid

#190434

Purpose

M13 packaging signal cloned into the pSB1K3 vector.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 packaging signal

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pC1.530

Plasmid

#205441

Purpose

Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

RepB gRNA

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAS007

Plasmid

#164868

Purpose

This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.

Depositor

Keith Shearwin

Article

Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

Insert

phiKO2 operators

Use

Synthetic Biology

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJR2953

Plasmid

#122549

Purpose

Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemCardinal2

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pWT062d

Plasmid

#107911

Purpose

W2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

H1-TetR-sgRNA B

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRPR1_c4gRNA_RPR1t

Plasmid

#64382

Purpose

encodes c4 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c4 gRNA

Expression

Yeast

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c8gRNA_RPR1t

Plasmid

#64386

Purpose

encodes c8 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c8 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only