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pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

Plasmid

#134339

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Spse

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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BRWD1_pCSdest

Plasmid

#53894

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

BRWD1 (BRWD1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pSN65

Plasmid

#100123

Purpose

expresses putative SOX2 DBD with a V5 tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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CHODL_pCSdest

Plasmid

#53793

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CHODL (CHODL Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
gh42

Plasmid

#106716

Purpose

expression of gRNA targeting ST6GALNAC1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GALNAC1 (ST6GALNAC1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh45

Plasmid

#106708

Purpose

expression of gRNA targeting ST6GALNAC4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GALNAC4 (ST6GALNAC4 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shId2 #1

Plasmid

#83088

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCNL-C1_RhoB

Plasmid

#65697

Purpose

Expresses CNL-RhoB in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

RhoB (RHOB Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only
pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128342

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna7 (Chrna7 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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8xCTS1_delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

Plasmid

#124545

Purpose

Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro

Expression

Mammalian

Mutation

d11-41, d11-41

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2317

Plasmid

#36743

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FGFR1-TALEN_Right (FGFR1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2316

Plasmid

#36742

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FGFR1-TALEN_Left (FGFR1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only
pSicoR (Puro) NEMO shRNA1

Plasmid

#22505

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA1 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pSN20

Plasmid

#100105

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'medium' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-WIP 460 deletion

Plasmid

#11773

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP aa1-460 (WIPF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

aa461-503 deleted

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-FLEX-T-RG

Plasmid

#102368

Purpose

Express cre dependent TVA and SADB19 RG (biscistronic) as an AAV

Depositor

Troy Margrie

Article

Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

Insert

TVA E2A-RG

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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gh242

Plasmid

#106850

Purpose

expression of gRNA targeting PIGA

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

PIGA (PIGA Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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sgRNA4_NANOG

Plasmid

#64156

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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SYNJ1-170_pCSdest

Plasmid

#53822

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SYNJ1-170 (SYNJ1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
Zebrafish-gRNA-0002

Plasmid

#42242

Purpose

gRNA targeted to zebrafish gene drd3

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-drd3 (drd3 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only