202,472 results

1. pInt-ts

   Plasmid

   #66076

   Purpose

   The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   lambda integrase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pInt-ts

   Plasmid

   #66076

   Purpose

   The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   lambda integrase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFD6

   Plasmid

   #73915

   Purpose

   tissue-specific multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   :tRNA:gRNA:tRNA:gRNA:tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAS-DSCP

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSP2-96-merTetO-EFS-BLaR

   Plasmid

   #118713

   Purpose

   Contains an optimized 96-mer TetO repeat for imaging of desired loci

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Inserts

   Blasticidin resistance gene
   EFS-NS promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PB_rtTA_BsmBI

   Plasmid

   #126028

   Purpose

   PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-Cre-P2A-dTomato (AAV PHP.eB)

   Viral Prep

   #107738-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Rylan Larsen

   Promoter

   Syn

   Tags

   dTomato (physically separate, not a fusion protein)

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

7. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Use

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

   Plasmid

   #44361

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

   Plasmid

   #44361

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBPGAL80Uw-6

    Plasmid

    #26236

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge