We narrowed to 1,485 results for: asl

1. pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

   Plasmid

   #217762

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Linker GGSSGGGA plus C20 domain (aa 405-592)

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEGFP-ORP8-H514A-H515A

   Plasmid

   #208360

   Purpose

   Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

   Depositor

   Paula Nunes-Hasler

   Article

   Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

   Insert

   ORP8 (OSBPL8 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H514A-H515A

   Promoter

   CMV

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET19-TdTc253

   Plasmid

   #207464

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc253 (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET19-TdTc188

   Plasmid

   #207463

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc188 (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET19-TdTc180

   Plasmid

   #207462

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc180 (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET19-TdTc302

   Plasmid

   #207461

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc302 (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Sec22b-P33-shR

   Plasmid

   #208359

   Purpose

   Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

   Depositor

   Paula Nunes-Hasler

   Article

   Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

   Insert

   Sec22b (Sec22b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   four silent mutations (shRNA resistance), 33 aa p…

   Promoter

   CMV

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-3xNLS-OptoJNKi-dsm

   Plasmid

   #89743

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi dark-state mutant
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C450A mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-3xNLS-OptoJNKi-lsm

   Plasmid

   #89742

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi lit-state mutant
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   I539E mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLuc-Optop38i-lsm

    Plasmid

    #89747

    Purpose

    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i lit-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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