Addgene

We narrowed to 3,514 results for: IPP

Showing: 721 - 740 of 3514 results

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pAAV hSyn INTRON mCherry-Jph3 WPRE

Plasmid

#236237

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

Plasmid

#236245

Purpose

Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Expression

Mutation

Promoter

U6 and human Synapsin1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

Plasmid

#236240

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.2 (CACNA1B Rabbit)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-Sec61b WPRE

Plasmid

#236228

Purpose

AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Sec61b (SEC61B Human, Synthetic)

Use

AAV

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha

Plasmid

#181895

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha (Cbx5 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha-delCTE

Plasmid

#181896

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha-delCTE (Cbx5 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-FUSN-mHP1alpha

Plasmid

#181897

Purpose

Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

FUSN-mHP1alpha (Cbx5 Mouse, Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-PGL-1-mHP1alpha

Plasmid

#181899

Purpose

Fluorescently tagged HP1alpha fused to PGL-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PGL-1-mHP1alpha (Cbx5 Mouse, Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRGG-GFP-RGG-mHP1alpha

Plasmid

#181898

Purpose

Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

RGG-GFP-RGG (Cbx5 Mouse, Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4(TA)-ires-blast

Plasmid

#167831

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hHP1β

Plasmid

#103829

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hHP1β (CRY2, CBX1 Mustard Weed, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5mut

Plasmid

#103828

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5mut (CRY2, KAT2A Mustard Weed, Human)

Use

Tags

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5-NLS

Plasmid

#103827

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5-NLS (CRY2, KAT2A Mustard Weed, Human)

Use

Tags

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5

Plasmid

#103826

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5 (CRY2, KAT2A Mustard Weed, Human)

Use

Tags

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11

Plasmid

#164203

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald D40G

Plasmid

#164211

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

D40G

Promoter

EF1a

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(D40G)

Plasmid

#164204

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

D40G

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)

Plasmid

#164208

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

186-505 aa

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(1-185aa)

Plasmid

#164207

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

1-185 aa

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-mHP1alpha

Plasmid

#181883

Purpose

Soluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-mHP1alpha (Cbx5 Mouse, Mustard Weed)

Use

Tags

PHR-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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