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Plasmid#81110PurposeGene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
300_pETcon_SARS2_FLip
Plasmid#222230Purposeyeast surface display of the SARS-CoV-2 FLip variant RBDDepositorInsertSARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
297_pETcon_SARS2_Omicron-BA286
Plasmid#222231Purposeyeast surface display of the SARS-CoV-2 BA.2.86 variant RBDDepositorInsertSARS-CoV-2 BA.2.86 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
299_pETcon_SARS2_EG-5
Plasmid#222232Purposeyeast surface display of the SARS-CoV-2 EG.5 variant RBDDepositorInsertSARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-tepsin
Plasmid#196647PurposeExpression of GAL4 DNA-binding domain (BD)-tepsin fusion proteinDepositorInserttepsin (TEPSIN Human)
UseYeast two-hybrid vectorTagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 199 to eliminate int…PromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETCON-Aga2p-TAPBPR-myc
Plasmid#153486PurposeYeast expression of Aga2p-TAPBPR-mycDepositorInsertTAPBPR (TAPBPL Human)
UseTagsAga2P and c-myc epitope tagExpressionYeastMutationPromoterGALAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-G96C/A97C/U100C/U101C
Plasmid#82328PurposeU6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-A34G/A35G/G96C/A97C
Plasmid#81238PurposeU6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G/G96C/A97C mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C)PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(G:H)
Plasmid#138257PurposeExpression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter systemDepositorInsertGFP targeting sgRNAs G and H
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTJK504
Plasmid#121459PurposeCEN HIS3 CNB1mutEF2DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 66 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSN067
Plasmid#101748PurposeSingle copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZJOM43
Plasmid#133652PurposeIntegration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-VPS4-DuDre (VPS4 Budding Yeast)
UseTagsDuDreExpressionYeastMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM149
Plasmid#133646PurposeIntegration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorInsertp1k-ELO3-mCherry (ELO3 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM44
Plasmid#133653PurposeIntegration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-SNF7-mCherry (SNF7 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSN068
Plasmid#101749PurposeSingle copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM70
Plasmid#133658PurposeIntegration of ERG6-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.DepositorInsertp1k-ERG6-mCherry (ERG6 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM113
Plasmid#133666PurposeIntegration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYX233
Plasmid#163762PurposeEmpty vector for Gal-inducible yeast expressionDepositorTypeEmpty backboneUseTagsHemagglutinin (HA) tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
3413 pBF339 Bcl-XL
Plasmid#8773DepositorInsertBcl-XL (Bcl2l1 Mouse)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
ZJOM136
Plasmid#133660PurposeIntegration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorInsertp1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only