We narrowed to 5,513 results for: mcs

1. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

   Plasmid

   #177265

   Purpose

   STREAMING-tag cassette

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag cassette
   

   Use

   Unspecified

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CBh-mKate2-IRES-MCS (WT-WT) (AAV2)

   Viral Prep

   #105921-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-CBh-mKate2-IRES-MCS (WT-WT) (#105921). In addition to the viral particles, you will also receive purified pAAV-CBh-mKate2-IRES-MCS (WT-WT) plasmid DNA. Control mKate2 expression AAV with two wild-type ITR sequences. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Marcella Patrick

   Promoter

   CBh and same CBh promoter as mKate2

   Tags

   mKate2

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pLPC FLAG hRap1 delta MC

   Plasmid

   #13254

   Depositor

   Titia de Lange

   Article

   Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

   Insert

   Repressor Activator Protein 1 delta MC (TERF2IP Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Mutation

   contains hRap1 aa2-131 + 265-399 and the point mu…

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pACE-GIRK4-mC-S

   Plasmid

   #172427

   Purpose

   Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK4 (KCNJ5 Human)
   

   Tags

   mCherry Streptag II

   Expression

   Insect

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV dCas9-MC (eGFP)

   Plasmid

   #89931

   Purpose

   Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deactivated Cas9, fragment of M.SssI (residues 27…

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPIC-mouseKir3.2-mC-S

   Plasmid

   #140745

   Purpose

   Expression of mouse Kir3.2 fused to mCherry and Streptag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

   Insert

   Kir3.2 (Kcnj6 Mouse)
   

   Tags

   mCherry with Streptag II

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACE-GIRK2-mC-S

   Plasmid

   #172428

   Purpose

   Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK2 (KCNJ6 Human)
   

   Tags

   mCherry Streptag II

   Expression

   Insect

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDOE20 mC VN transient

   Plasmid

   #124247

   Purpose

   Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.

   Depositor

   Attila Fehér

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

   Plasmid

   #117005

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC FLAG hRap1 delta MC

    Plasmid

    #13254

    Depositor

    Titia de Lange

    Article

    Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

    Insert

    Repressor Activator Protein 1 delta MC (TERF2IP Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Mutation

    contains hRap1 aa2-131 + 265-399 and the point mu…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pCDH-CMV-mC-G-LC3B-P

    Plasmid

    #124974

    Purpose

    evaluate autophagy

    Depositor

    Kazuhiro Oka

    Article

    Sutton et al Autophagy. 2018;14(4):637-653. doi: 10.1

    Insert

    mCherry-GFP-LC3B
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AV0-BWS-2.0-mC-G-CL3B

    Plasmid

    #124975

    Purpose

    evaluate autophagy

    Depositor

    Kazuhiro Oka

    Insert

    mCherry-GFP-LC3B
    

    Use

    AAV

    Promoter

    EF1-alpha

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Murine cell surface protein knockout (McspKO) library

    Pooled Library

    #200445

    Purpose

    A CRISPR library targeting 1,157 cell surface genes and 98 control genes with four sgRNAs per gene

    Depositor

    Junjie Chen

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 Jun 21;11

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pBluescript II NLS-MC-EGFP-tdTomato (pBET2)

    Plasmid

    #240411

    Purpose

    This plasmid facilitates the assessment of MMR proficiency in human living cells

    Depositor

    Isao Kuraoka

    Article

    Takedachi et al Mutat Res. 2022 Jan-Jun;824:111779. doi:

    Insert

    NLS-EGFP fusion protein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

    Plasmid

    #156416

    Purpose

    kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12V (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    mScarlet-I

    Mutation

    mutant KRAS4A-G12V

    Promoter

    kdrl

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. wt 6XHis-SNX9 pET15b

    Plasmid

    #34690

    Depositor

    Sandra Schmid

    Article

    Yarar et al Traffic. 2008 Jan;9(1):133-46. Epub 2007

    Insert

    Sorting nexin 9
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pHIV-Luciferase

    Plasmid

    #21375

    Purpose

    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-Akt DN (K179M)

    Plasmid

    #16243

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al J Biol Chem. 2000 Mar 17. 275(11):8027-3

    Insert

    Akt1 (Akt1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative (K179M)

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-TRIM24-C840W

    Plasmid

    #28136

    Depositor

    Michelle Barton

    Article

    Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

    Insert

    TRIM24 (TRIM24 Human)
    

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    A489V and C840W

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

20. Flag-TRIM24-C840W shRescue

    Plasmid

    #28137

    Depositor

    Michelle Barton

    Article

    Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

    Insert

    TRIM24 (TRIM24 Human)
    

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    C840Wmutated at aa 308 and 309 for shRNA rescue; …

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only