We narrowed to 7,098 results for: poly

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pGEX4T2 RANK-L

Plasmid

#108571

Purpose

Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

Depositor

Jean-Claude Scimeca

Article

Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

Insert

RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)

Tags

GST

Expression

Mammalian

Mutation

aa158-aa316

Promoter

Ptac (trp/lac)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V3

Plasmid

#226956

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 GFP V3

Plasmid

#226961

Purpose

CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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PDG459 V3

Plasmid

#226958

Purpose

CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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SaCas9 GFP V3

Plasmid

#226962

Purpose

CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1-3xFlag-7xKQ

Plasmid

#111576

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

lysine to glutamine mutation at the following ami…

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX2-1-178-EGFP-Hygro

Plasmid

#238094

Purpose

Expression of structurally predicted WWE domain of DTX2 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX2 (DTX2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-178 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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SaCas9v2-Puro

Plasmid

#178802

Purpose

SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PB-iRFP-STOP-ReNL

Plasmid

#113965

Purpose

Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Inserts

iRFP670
ReNL

Use

Piggybac transposon

Expression

Mammalian

Promoter

CMV - SV40-PolyA - SV40-PolyA and EF1A

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP11-22-122-EGFP-Hygro

Plasmid

#238097

Purpose

Expression of structurally predicted WWE domain of PARP11 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP11 (PARP11 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 22-112 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP12-298-462-Hygro

Plasmid

#238098

Purpose

Expression of structurally predicted WWE domain of PARP12 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP12 (PARP12 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 298-462 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DDHD2-30-202-Hygro

Plasmid

#238101

Purpose

Expression of structurally predicted WWE domain of DDHD2 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DDHD2 (DDHD2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 30-202 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-RNF146-45-182-EGFP-Hygro

Plasmid

#238091

Purpose

Expression of structurally predicted WWE domain of RNF146 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146 (RNF146 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 45-182 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-TRIP12-391-938-EGFP-Hygro

Plasmid

#238092

Purpose

Expression of structurally predicted WWE domain of TRIP12 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

TRIP12 (TRIP12 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 319-938 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX1-1-175-EGFP-Hygro

Plasmid

#238093

Purpose

Expression of structurally predicted WWE domain of DTX1 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX1 (DTX1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-175 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX4-1-166-EGFP-Hygro

Plasmid

#238095

Purpose

Expression of structurally predicted WWE domain of DTX4 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX4 (DTX4 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-166 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-HUWE1-1503-1780_EGFP-Hygro

Plasmid

#238096

Purpose

Expression of structurally predicted WWE domain of HUWE1 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

HUWE1 (HUWE1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-166 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc

Plasmid

#196237

Purpose

Af1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

Af1521(K35E/Y145R) encoding a C-terminal myc tag

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

amino acid 35 lysine is replaced with glutamic ac…

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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yTREX-ApR

Plasmid

#177307

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V2

Plasmid

#226955

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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