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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 29,164 results for: eng

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Showing: 7421 - 7440 of 29164 results

  1. pX330A_D10A-1x6

    Plasmid
    #58776
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58776-plasmid-map-sequence-id-91086

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  5. ttAtCas12a+int

    Plasmid
    #182384
    Purpose
    Gives very high editing efficiency in barley. Also works in Brassica oleracea
    Depositor
    Wendy Harwood
    Publication
    Lawrenson et al bioRxiv 2022.04.28.489853
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a coding sequence with D156R and Arabidopsis introns
    Use
    CRISPR
    Mutation
    D156R + 8 Arabidopsis introns added
    Promoter
    None
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182384-plasmid-map-sequence-id-361472

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  8. pX330A_D10A-1x6

    Plasmid
    #58776
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58776-plasmid-map-sequence-id-91086

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  13. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid
    #197028
    Purpose
    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197028-plasmid-map-sequence-id-388968

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  16. pCEP4-YU2gp160

    Plasmid
    #100927
    Purpose
    Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (YU2) Env
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100927-plasmid-map-sequence-id-459467

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  17. pCAG-mCherry-FKBP-GEF(TIAM1)

    Plasmid
    #85156
    Purpose
    acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB
    Depositor
    Tobias Meyer
    Publication
    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
    Tags
    mCherry-FKBP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85156-plasmid-map-sequence-id-166559

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  18. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid
    #197028
    Purpose
    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197028-plasmid-map-sequence-id-388968

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Showing: 7421 - 7440 of 29164 results