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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,073 results for: CAG

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Showing: 7441 - 7460 of 10073 results

  13. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid
    #208979
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208979-plasmid-map-sequence-id-418375

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  15. BPK1520-LRRK2-G2019S-ng

    Plasmid
    #180436
    Purpose
    Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach
    Depositor
    Frank Soldner
    Publication
    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180436-plasmid-map-sequence-id-357728

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  16. pX459-HypaCas9-mR2-RHOA_sgRNA

    Plasmid
    #183877
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RHOA sgRNA spacer (RHOA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183877-plasmid-map-sequence-id-362410

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  18. pEBTetBl-CLIP-CCCAP

    Plasmid
    #136847
    Purpose
    Mammalian expression of the centrosomal protein CCCAP N-terminally fused to CLIP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CLIP-CCCAP (SDCCAG8 Human, Synthetic)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    136847-plasmid-map-sequence-id-276286

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  19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide3

    Plasmid
    #118175
    Purpose
    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118175-plasmid-map-sequence-id-240390

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Showing: 7441 - 7460 of 10073 results