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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,731 results for: EMB

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Showing: 7441 - 7460 of 11731 results

  1. TP1331

    Plasmid
    #252364
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC nanobody D2
    Tags
    ALFA-sfGFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252364-plasmid-map-sequence-id-504593

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  2. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid
    #236245
    Purpose
    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    Use
    AAV
    Tags
    mTagBFP2
    Promoter
    U6 and human Synapsin1
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236245-plasmid-map-sequence-id-471239

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  6. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  7. pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

    Plasmid
    #168152
    Purpose
    Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    Expression
    Bacterial
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168152-plasmid-map-sequence-id-330917

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  8. pGBW-m4046688

    Plasmid
    #149185
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149185-plasmid-map-sequence-id-296388

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  9. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

    Plasmid
    #114144
    Purpose
    The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
    Expression
    Mammalian
    Mutation
    G2A mutation and silent mutation to resist shRNA …
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114144-plasmid-map-sequence-id-224274

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  17. pGBW-m4046240

    Plasmid
    #149144
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149144-plasmid-map-sequence-id-298243

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  18. pGBW-m4046253

    Plasmid
    #149135
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149135-plasmid-map-sequence-id-307321

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  19. pGBW-m4046225

    Plasmid
    #149058
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149058-plasmid-map-sequence-id-297939

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  20. CFFΔC-EGFP

    Plasmid
    #86059
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains CD8a luminal and transmembrane domains a…
    Promoter
    CMV
    Available Since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    86059-plasmid-map-sequence-id-179753

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Showing: 7441 - 7460 of 11731 results