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Pr1-T7RNAP

Plasmid

#67738

Purpose

OR2-OR1-Pr1 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr1

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PPARG

Plasmid

#67099

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PPARG (PPARG Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 PPARG

Plasmid

#67099

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PPARG (PPARG Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Cdk3-DN

Plasmid

#1879

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk3 (CDK3 Human)

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
Cdk3-DN

Plasmid

#1879

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk3 (CDK3 Human)

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
mEos2-Caveolin-C-10

Plasmid

#57355

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Caveolin (CAV1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-Caveolin-C-10

Plasmid

#57355

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Caveolin (CAV1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-Actin-C-18

Plasmid

#57340

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Actin-C-18

Plasmid

#57340

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCellFree_G03 PSMC1

Plasmid

#67087

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PSMC1 (PSMC1 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

E275G, I287V and R294G

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 PSMC1

Plasmid

#67087

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PSMC1 (PSMC1 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

E275G, I287V and R294G

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-D149A

Plasmid

#29351

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Asp149Ala

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-D149A

Plasmid

#29351

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Asp149Ala

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pTK_Cerulean_Nanotag_66_neg_Control

Plasmid

#130567

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_62_neg_Control

Plasmid

#130565

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Citrine_Nanotag_35_neg_Control

Plasmid

#130564

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Cerulean_Nanotag_92

Plasmid

#130540

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_71

Plasmid

#130533

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Cerulean_Nanotag_62

Plasmid

#130530

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_61

Plasmid

#130529

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge