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p3E-P2A-CFP no-pA

Plasmid

#80827

Purpose

P2A followed by CFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-CFP no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAEF5

Plasmid

#136305

Purpose

Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

Depositor

Gilles Fischer

Article

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Type

Empty backbone

Use

CRISPR

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Twitch-1 pRSETB

Plasmid

#48207

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mues et al Nat Med. 2013 Jun;19(6):778-83. doi: 10.

Insert

Twitch-1

Tags

6xHIS, ECFP, and cpCit174

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only
pEpic

Plasmid

#84372

Purpose

3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSB19-PapRS#34-OmpA

Plasmid

#231105

Purpose

Vector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)

Depositor

Arne Skerra

Article

Mayrhofer et al Nat Commun. 2024 Dec 18;15(1):10693. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSynapsin flexed SV-tag

Plasmid

#163686

Purpose

express SV-Tag construct in a Cre-dependent manner

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

synaptophysin 9X HA

Use

AAV

Tags

9X HA

Expression

Mammalian

Promoter

synapsin

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-lynD3cpV

Plasmid

#37472

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Tags

the myristoylation and palmitoyla- tion sequence …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
p6643 MSCV-P C-FlagHA 31E7-Kozak

Plasmid

#35021

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV31 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
MCV-HF

Plasmid

#32057

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-HF genome

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS20

Plasmid

#48342

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS20

Tags

TN-XXL

Expression

Mammalian

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pCMU-TLEr

Plasmid

#61187

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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LP376

Plasmid

#90200

Purpose

pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC10 (TRAPPC10 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1000-1259

Promoter

CMV

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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Bongwoori-R3

Plasmid

#101658

Purpose

A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-R3

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP B56 gamma3 shRNA 14-1

Plasmid

#10681

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma3 shRNA (PPP2R5C Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCMB-TPr

Plasmid

#61190

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pQTEV-PEPD

Plasmid

#31328

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

peptidase D (PEPD Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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5HT6-CFP-Venus(H148G)

Plasmid

#47501

Purpose

Fluorescent reporter for pH inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

Venus(H148G)

Tags

5HT6 and CFP

Expression

Mammalian

Mutation

H148G mutation

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCAGGS-GCaMP2-actin

Plasmid

#18928

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2-Actin (ACTB Chicken)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pJH3377

Plasmid

#175286

Purpose

Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16 d/f (daf-16 Nematode)

Expression

Worm

Promoter

Pges-1

Available Since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only