We narrowed to 17,340 results for: EMB

1. Expression scaffold for dCas9+sgRNA

   Plasmid

   #171799

   Purpose

   PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Insert

   PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
   

   Use

   CRISPR

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOGG010

   Plasmid

   #113984

   Purpose

   Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   RK2 (IncP)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLSU4cg10

   Plasmid

   #126078

   Purpose

   Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   Chloroplastic FNR transit peptide
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Promoter

   longCaMV35S

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDendra2-H2a

   Plasmid

   #157816

   Purpose

   Fluorescent reporter for histone H2A tracking

   Depositor

   Xin Chen

   Article

   Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

   Insert

   H2A (H2ac13 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTE104

   Plasmid

   #59399

   Purpose

   MethyloBrick expression vector, coxB promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PcoxB

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-ChETA-mRuby2-ST

   Plasmid

   #109126

   Purpose

   Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ChETA-mRuby2-ST
   

   Expression

   Mammalian

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFP.R364

   Plasmid

   #138234

   Purpose

   Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFP.R274

   Plasmid

   #138223

   Purpose

   Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GSK).TvoVMA.(TVI)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL-L1-1.3-KRAB

   Plasmid

   #103034

   Purpose

   [Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domain

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

   Insert

   TAL-L1-1.3-KRAB
   

   Use

   T3 in vitro transcription for expression in mamma…

   Tags

   HA and KRAB

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMaCTag-Z21

    Plasmid

    #120064

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    24xSunTag
    

    Use

    Pcr template

    Tags

    24xSunTag

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRE:shRNA A-pGK:rtTA-Cre

    Plasmid

    #68471

    Purpose

    Inducible lentivirus expressing an arbitrary shRNA and Cre

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    GFP-shRNA A-miR30
    rtTA-2A-Cre
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    pGK and pTRE

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. OPN(-213)-luc

    Plasmid

    #31107

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    osteopontin promoter (SPP1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    promoter region -213 to +87

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-RhebL1

    Plasmid

    #133766

    Purpose

    Expresses GFP-tagged RhebL1 in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    RhebL1 (RHEBL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMB-NUCr

    Plasmid

    #61169

    Purpose

    Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    NLS-mCherry-GUS
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    MtBCP1

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDDGFP-Leu2d-ScVrg4

    Plasmid

    #102334

    Purpose

    Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

    Insert

    YGL225W (VRG4 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL2-GGA(EAAAK)2AGG

    Plasmid

    #137104

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    GGA(EAAAK)2AGG
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TOPO pTRE

    Plasmid

    #68457

    Purpose

    TOPO construct expressing pTRE for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Tetracycline Response Element Promoter
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRU323

    Plasmid

    #167690

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-FLAG-GFP-Ubl4A

    Plasmid

    #86921

    Purpose

    To express Ubl4A tagged with FLAG-EGFP at N- terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    UBL4A (UBL4A Human)
    

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. hSERT-N1-pECFP

    Plasmid

    #70106

    Purpose

    mammalian expression of hSERT with C-terminal CFP fusion

    Depositor

    Harald Sitte

    Article

    Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

    Insert

    SERT (SLC6A4 Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only