We narrowed to 9,930 results for: pho;

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tet-pLKO shDUSP19 puro

Plasmid

#87796

Purpose

Expresses an inducible short hairpin targeting human DUSP19 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shDUSP19 (DUSP19 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP7.v2 puro

Plasmid

#87795

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

Plasmid

#87776

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4 (MAP2K4 Human)

Tags

3xFLAG and NLS

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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RSV/mGATA3 (antisense)

Plasmid

#83817

Purpose

contains antisense mouse GATA3

Depositor

Douglas Engel

Article

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

GATA3-antisense strand (Gata3 Mouse)

Expression

Mammalian

Promoter

RSV

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
Sema3f(L,-)-AP-His

Plasmid

#72022

Purpose

Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains a 93 nt deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3f(S,-)-AP-His

Plasmid

#72024

Purpose

Expresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains a 93 nt deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3b(S)-AP-His

Plasmid

#72014

Purpose

Expresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3b (Sema3b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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213-petDuet1-GBD-LUMP--cdc42

Plasmid

#65973

Purpose

Dual expression of GBD-LUMP and cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Inserts

LUMP
cell division cycle 42 (CDC42 Human)

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Mutation

constitutively active Cdc42 (Q61L)

Promoter

T7

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase delta 1278-1291

Plasmid

#59830

Purpose

Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 1278-1291

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

Plasmid

#58490

Purpose

Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

Depositor

Adam Cohen

Article

Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

Insert

Archaerhodopsin-3 w/ D95H point mutation fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Glutamine

Promoter

Ubiquitin

Available Since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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Comp AlphaPS3

Plasmid

#14071

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Comp Alpha PS3 Integrin (scb Fly)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pLENTICRISPR-NDUFB5-sgRNA1

Plasmid

#165939

Purpose

CRISPR-mediated gene perturbation (knock-out) of NDUFB5

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

NDUFB5 (NDUFB5 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-NDUFB5-sgRNA2

Plasmid

#165940

Purpose

CRISPR-mediated gene perturbation (knock-out) of NDUFB5

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

NDUFB5 (NDUFB5 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-PGAM1-sgRNA1

Plasmid

#165943

Purpose

CRISPR-mediated gene perturbation (knock-out) of PGAM1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PGAM1 (PGAM1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-PGAM1-sgRNA2

Plasmid

#165944

Purpose

CRISPR-mediated gene perturbation (knock-out) of PGAM1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PGAM1 (PGAM1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRNP70-sgRNA1

Plasmid

#165945

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRNP70

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRNP70 (SNRNP70 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRNP70-sgRNA2

Plasmid

#165946

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRNP70

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRNP70 (SNRNP70 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-TPI1-sgRNA1

Plasmid

#165949

Purpose

CRISPR-mediated gene perturbation (knock-out) of TPI1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-TPI1-sgRNA2

Plasmid

#165950

Purpose

CRISPR-mediated gene perturbation (knock-out) of TPI1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-puro-PFKM

Plasmid

#165953

Purpose

Overexpression of PFKM.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PFKM (PFKM Human)

Use

Lentiviral

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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